Strep-Tactin® Suspension

The following resins are provided as 50 % suspensions:
  • Strep-Tactin® Sepharose®
  • Strep-Tactin® Superflow®
  • Strep-Tactin® Superflow® high capacity
  • Strep-Tactin® MacroPrep®
When to use which resin?
Sepharose®
  • Economic alternative to Superflow®
  • Agarose support with good flow properties
  • For gravity flow chromatography only (not pressure stable)

Superflow®

  • Our all-rounder and the resin to start with for large proteins and protein:protein interaction analysis
  • Agarose support with low unspecific binding properties
  • For increased flow rates
  • Low pressure stable (FPLC/HPLC/Äkta applications)

Superflow® high capacity (HC)

  • The capacity of Superflow® is not sufficient or the binding affinity to Twin-/Strep-tag® fusion
  • proteins needs to be slightly improved!
  • 3-5 fold higher binding capacity as Superflow®
MacroPrep®
  • The alternative if Sepharose® and Superflow® lead to non-specific binding of protein (e.g. Chlorophyll from plant extracts)
  • Synthetic support with low non-specific binding propertie
  • Highly pressure stable (FPLC/HPLC /Äkta applications)

 

These products can be used to pack your own columns. We provide them in different sizes:

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
10x Buffer E; Strep-Tactin® Elution Buffer with Desthiobiotin 25 ml 2-1000-025 USD 62.00  
2-1000-025

Buffer E is a Strep-Tactin® Elution Buffer with Desthiobiotin (10x concentrated) and used for elution of Strep-tag®II and Twin-Strep-tag® proteins from the biotin-binding site of Strep-Tactin® resins in a competitive manner resulting in a mild elution. ...

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Strep-Tactin® Purification Buffer Set 1 Set 2-1002-001 USD 87.00  
2-1002-001

Strep-Tactin® Purification Buffer Set is a convenient buffer set for the purification of recombinant Strep-tag®II and Twin-Strep-tag®II fusion proteins on immobilized Strep-Tactin® and its regeneration. ...

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Buffer R; Strep-Tactin® Regeneration Buffer with HABA; 100 ml 2-1002-100 USD 50.00  
2-1002-100

Buffer R is a Strep-Tactin® Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin®. HABA (2-[4 -hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin® Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin®. The yellow HABA solution turns red upon binding to Strep-Tactin® clearly indicating the regeneration process and activity status of the column. ...

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10x Buffer W; Strep-Tactin®/Strep-Tactin®XT Wash Buffer 100 ml 2-1003-100 USD 43.00  
2-1003-100

Strep-Tactin®/Strep-Tactin®XT Wash Buffer is used for the removal of unbound molecules from immobilized Strep-Tactin® and Strep-Tactin®XT during Strep-tag® protein purification.

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GFP-Strep-tag control protein 500 µg of a 28 kDa protein 2-1006-005 USD 109.00  
GFP-Twin-Strep-tag control protein 500 µg of a 30 kDa protein 2-1007-005 USD 109.00  
Strep-Tactin® Sepharose® 50% suspension 4 ml 2-1201-002 USD 117.00  
2-1201-002

Strep-Tactin® Sepharose® is used preferentially for gravity flow purification of recombinant Strep-tag® proteins. Bulk resin is recommended for packing of our columns and cartridges since the resin is optimized for column affinity chromatography rather than for batch purification. ...

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Strep-Tactin® Sepharose® 50% suspension 20 ml 2-1201-010 USD 399.00  
2-1201-010

Strep-Tactin® Sepharose® is used preferentially for gravity flow purification of recombinant Strep-tag® proteins. Bulk resin is recommended for packing of our columns and cartridges since the resin is optimized for column affinity chromatography rather than for batch purification. ...

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Strep-Tactin® Sepharose® 50% suspension 50 ml 2-1201-025 USD 949.00
2-1201-025

Strep-Tactin® Sepharose® is used preferentially for gravity flow purification of recombinant Strep-tag® proteins. Bulk resin is recommended for packing of our columns and cartridges since the resin is optimized for column affinity chromatography rather than for batch purification. ...

Further details, manuals, data sheets, related products and more
Strep-Tactin® Sepharose® 50% suspension 1000 ml 2-1201-500    
 

Strep-Tactin® Sepharose® is used preferentially for gravity flow purification of recombinant Strep-tag® proteins. Bulk resin is recommended for packing of our columns and cartridges since the resin is optimized for column affinity chromatography rather than for batch purification. ...

Further details, manuals, data sheets, related products and more