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Strep-Tactin® TACS Agarose

50% suspension for traceless affinity cell and exosome isolation
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From 5
Strep-Tactin® TACS Agarose is suitable for cell selection or depletion via affinity chromatography. The resin consists of agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the optimal use for cell-related approaches. 
Strep-Tactin® TACS Agarose can be loaded with a protein of choice that is fused to a Twin-Strep-tag®. This can be for example an antibody or an antigen, which captures the cells inside the columns. Biotin addition causes the dissociation of strep-tagged proteins from Strep-Tactin®, leading to the elution of cells.
Alternatively, it is possible to load the resin with Twin-Strep-tagged, desthiobiotinylated or biotinylated proteins (irreversible binding) for negative cell selection or depletion. 
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.


Biotin Binding Capacity: > 40 nmol/ml resin
Form: 50% suspension
Possible Application: Cell isolation or depletion via affinity chromatography
Support: Crosslinked agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature


Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
Buffer CI (10x)
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA

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Gravity flow column suitable for traceless affinity cell and exosome isolation
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