6-6350-010

Strep-Tactin® TACS Agarose

50% suspension for traceless affinity cell and exosome isolation

€339.00*

Content
Strep-Tactin® TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. It was developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The eluted cells are ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin. This makes Strep-Tactin® TACS Agarose also suitable for negative cell selection or depletion approaches. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.

Specifications

Biotin Binding Capacity: > 40 nmol/ml resin
Form: 50% suspension
Possible Application: Affinity chromatographic cell or exosome isolation
Support: Crosslinked agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
10x Buffer CI
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA

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Strep-Tactin® multimers conjugated with magnetic microbeads
Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
Strep-Tactin® PE or APC
Fluorophore-conjugated Strep-Tactin® for cell staining