2-1564-050
StrepMAB-Immo DY-488 conjugate
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
| Quantity | Unit price |
|---|---|
| 1-1 |
€291.00*
|
| 2-4 |
€276.00*
|
| From 5 |
€261.50*
|
Content
StrepMAB-Immo DY-488 is a murine, high-affinity monoclonal antibody, which is especially suited for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins in applications like western blots, ELISA, immunohistochemistry and immunocytochemistry.
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
The absorption/emission maxima of the fluorescent dye is:
• DY-488: 493/518 nm
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.
| Clonality: | Monoclonal |
|---|---|
| Concentration: | 0.5 mg/ml |
| Detector/Ligand: | Antibody |
| Form: | Suspension in PBS |
| Possible Application: | FACS, Immunofluorescence, ELISA |
| Specificity: | Strep-tag®II and Twin-Strep-tag® |
| Subclass: | IgG1 |
| Species: | Mouse |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
GFP-Twin-Strep-tag® control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
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