2-1517-001

StrepMAB-Immo

Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins

€337.00*

Content
StrepMAB-Immo is a murine, high-affinity monoclonal antibody, which is especially suited for stable, mild, and oriented capturing of Strep-tag®II and Twin-Strep-tag® fusion proteins on solid phases (e.g. Biacore CM5 sensor chips) or for detection of those proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry.
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing the Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.

Specifications

Clonality: Monoclonal
Form: Lyophilized powder
Possible Application: ELISA, FACS, Immunofluorescence, SPR
Reconstitution: PBS
Specificity: Strep-tag®II and Twin-Strep-tag®
Subclass: IgG1
Species: Mouse

Shipping information

Storage: -20 °C
Stability: 24 months after shipping
Shipping: Room temperature

Accessories

GFP-Strep-tag control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications

Related Products

StrepMAB-Immo conjugate
Monoclonal antibodies with different fluorescent conjugates to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
Strep-Tactin®
Reversible immobilization of biotinylated analytes or Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Classic
Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
Twin-Strep-tag® Capture Kit
Reversible capture of Twin-Strep-tag® fusion proteins for SPR analysis