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Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins


StrepMAB-Immo is a murine, high-affinity monoclonal antibody, which is especially suited for stable, mild, and oriented capturing of Strep-tag®II and Twin-Strep-tag® fusion proteins on solid phases (e.g. Biacore CM5 sensor chips) or for detection of those proteins in applications like ELISAFACS, immunohistochemistry and immunocytochemistry.
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing the Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.


Clonality: Monoclonal
Form: Lyophilized powder
Possible Application: ELISA, FACS, Immunofluorescence, SPR
Reconstitution: PBS
Specificity: Strep-tag®II and Twin-Strep-tag®
Subclass: IgG1
Species: Mouse

Shipping information

Storage: -20 °C
Stability: 24 months after shipping
Shipping: Room temperature

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