2-1517-001
StrepMAB-Immo
Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo is a murine, high-affinity monoclonal antibody, which is especially suited for stable, mild, and oriented capturing of Strep-tag®II and Twin-Strep-tag® fusion proteins on solid phases (e.g. Biacore CM5 sensor chips) or for detection of those proteins in applications like ELISA, FACS, immunohistochemistry and immunocytochemistry.
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing the Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing the Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.
| Clonality: | Monoclonal |
|---|---|
| Form: | Lyophilized powder |
| Possible Application: | ELISA, FACS, Immunofluorescence, SPR |
| Reconstitution: | PBS |
| Specificity: | Strep-tag®II and Twin-Strep-tag® |
| Subclass: | IgG1 |
| Species: | Mouse |
| Storage: | -20 °C |
|---|---|
| Stability: | 24 months after shipping |
| Shipping: | Room temperature |
Accessories
GFP-Twin-Strep-tag® control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
Related Products
Strep-Tactin®
Reversible immobilization of biotinylated analytes or Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-488 conjugate
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-549 conjugate
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-649 conjugate
Monoclonal antibody conjugated to DY-649 to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
