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2-1002-100

Buffer R (10x)

Strep-Tactin® regeneration buffer with HABA

€51.00*

Content: 1 Stk
Content
Buffer R (10x) is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA (2-[4'-hydroxy-benzeneazo]benzoic acid). HABA displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin® after protein elution. Upon binding of HABA to Strep-Tactin®, the yellow buffer turns red, indicating regeneration and column activity. Please note that 10x Buffer R cannot be used for the regeneration of Strep-Tactin®XT resins, but it can be used to monitor the resin's activity status after the regeneration.


Specifications

Concentration: 10x concentrated
Form: Solution
Possible Application: Regeneration of Strep-Tactin® resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin