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2-0204-050
Avidin
Efficient blocking of biotin and biotinlyated proteins
Avidin is a tetrameric biotin binding protein (KD ~1 x 10-14) extracted from egg white. It prevents biotin and biotinylated proteins from binding to Strep-Tactin® or Strep-Tactin®XT, whereas binding of the Twin-Strep-tag® and Strep-tag®II is not influenced. Biotin and/or biotinylated proteins can potentially reduce the binding capacity of Strep-Tactin® as well as Strep-Tactin®XT and are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag® and Strep-tag®II fusion proteins from biotin-containing extracts or media are intended to be purified via Strep-Tactin® or Strep-Tactin®XT affinity chromatography, biotin has to be masked by addition of avidin prior to application onto the resin.
Biotin Blocking Buffer (2-0501-002) for western blotting can be prepared by dissolving 2.5 mg/ml Avidin in PBS buffer.
| Form: | Powder |
|---|---|
| Possible Application: | Blocking of biotin in e.g. culture media |
| Size: | 17 kDa per subunit |
| Source: | Egg white |
| Specific Activity: | > 11 U/mg |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin® Sepharose® resin
Content:
4 ml
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Content:
1 ml
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
Content:
4 ml
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Content:
5 x 0.2 ml
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
Content:
1 ml
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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