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2-0205-050

BioLock

Biotin blocking solution for lysates and culture supernatants

€174.00*

Content: 1 Stk
Content
BioLock is a ready-to-use biotin blocking solution containing avidin. Avidin strongly binds to biotin and biotinylated proteins, but not to Strep-tag®II or Twin-Strep-tag®-fusion proteins. This way, unspecific binding of biotin/biotinylated proteins to Strep-Tactin® or Strep-Tactin®XT resins is prevented, ensuring an unimpaired binding capacity and a high purity of the target protein.
Biotin and/or biotinylated proteins are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag® and Strep-tag®II fusion proteins from biotin-containing extracts or media are intended to be purified, biotin has to be masked by addition of BioLock to the sample prior to applying it to the resin.
For masking biotin for western blot, we recommend using purified avidin.

Specifications

Form: Suspension
Possible Application: Blocking of biotin in e.g. culture media
Size: 17 kDa per subunit
Source: Egg white
Specific Activity: > 70 U/ml

Shipping information

Storage: Room temperature
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins