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Buffer R (10x)

Buffer R (10x) is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA (2-[4'-hydroxy-benzeneazo]benzoic acid). HABA displaces desthiobiotin from the modified biotin-binding pocket of Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity after protein elution. Upon binding of HABA to Strep-Tactin®, the yellow buffer turns red, indicating regeneration and column activity. Please note that 10x Buffer R cannot be used for the regeneration of Strep-Tactin® 4Flow® high capacity and Strep-Tactin®XT resins, but it can be used to monitor the resin's activity status after the regeneration.


Specifications

Concentration: 10x concentrated
Form: Solution
Possible Application: Regeneration of Strep-Tactin® Sepharose®, Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Related Products

Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer E (10x)
Strep-Tactin® elution buffer with desthiobiotin