2-1002-100
Buffer R (10x)
Strep-Tactin® regeneration buffer with HABA
Buffer R (10x) is a 10x concentrated Strep-Tactin® regeneration buffer containing HABA. HABA (2-[4'-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin after the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin®. The yellow buffer turns red upon binding of HABA to Strep-Tactin®, clearly indicating the regeneration process and activity status of the column.
Please note that Buffer R (10x) is not applicable for regeneration of Strep-Tactin®XT resins, but it can be used to monitor the activity status after the regeneration.
| Concentration: | 10x concentrated |
|---|---|
| Form: | Solution |
| Possible Application: | Regeneration of Strep-Tactin® resins |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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