Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.
Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin® or Strep-Tactin®XT chromatography, biotin must be masked by the addition of avidin prior to the application onto the column.
BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.
Add at least 1U of BioLock solution per µg of biotin.