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StrepMAB-Immo is a murine monoclonal antibody with a high affinity to Strep-tag® and Twin-Strep-tag® fusion proteins. It is suited for the immobilization of tagged proteins on solid phases (e.g. SPR sensor chips), as well as the detection in applications like ELISA, FACS, immunohistochemistry or immunocytochemistry.
Strep-tag® proteins with an N-terminal Serine-Alanine linker (protein-SA-WSHPQFEK or SA-WSHPQFEK-protein) are validated for nearly irreversible binding. Proteins with other N-terminal sequences bind with a reduced affinity. The Twin-Strep-tag® always carries the N-terminal SA linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in Western Blots, we recommend using StrepMAB-Classic or Strep-Tactin® conjugates.

Specifications

Clonality: Monoclonal
Form: Lyophilized powder
Possible Application: ELISA, FACS, Immunofluorescence, SPR
Reconstitution: PBS
Specificity: Strep-tag®II and Twin-Strep-tag®
Subclass: IgG1
Species: Mouse

Shipping information

Storage: -20 °C
Stability: 24 months after shipping
Shipping: Room temperature

Accessories

GFP-Strep-tagII control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
GFP-Twin-Strep-tag control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications

Related Products

StrepMAB-Classic
Monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
Strep-Tactin®
Reversible immobilization of biotinylated analytes or Strep-tag®II and Twin-Strep-tag® fusion proteins
Twin-Strep-tag® Capture Kit
Reversible capture of Twin-Strep-tag® fusion proteins for SPR analysis
StrepMAB-Immo DY-488
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-649
Monoclonal antibody conjugated to DY-649 to detect Strep-tag®II and Twin-Strep-tag® fusion proteins