5-4104-001

pASG-IBA104 vector

Periplasmic E. coli expression vector encoding an N-terminal Twin-Strep-tag®

€219.00*

Content
The pASG-IBA104 vector is designed for expression of recombinant proteins with an N-terminal Twin-Strep-tag® in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Twin-Strep-tag®
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: E. coli
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in E. coli
Promoter: tet Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 3997 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Anhydrotetracycline
Derivative of tetracycline for induction of tetracycline-controlled promoters
Competent E. coli TOP10
Chemically competent cells for plasmid propagation
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

Related Products

pASG-IBA102 vector
Periplasmic E. coli expression vector encoding a C-terminal Twin-Strep-tag®