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pASG-IBA105 vector
Cytoplasmic E. coli expression vector encoding an N-terminal Twin-Strep-tag®
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The pASG-IBA105 vector is designed for expression of recombinant proteins with an N-terminal Twin-Strep-tag® in E. coli.
The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
| Affinity Tag N'-terminal: | Twin-Strep-tag® |
|---|---|
| Cloning Method: | Direct cloning using restriction enzyme Esp3I |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Vector for recombinant expression in E. coli |
| Promoter: | tet Promoter |
| Resistance: | Ampicillin |
| Sequence: | See Documents |
| Size: | 3952 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
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