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5-4105-001

pASG-IBA105 vector

Please note that this product will be discontinued at the end of 2026.

€279.00*

Content: 1 Piece

Prices excl. VAT plus shipping costs

Content
Stk
Please note that this product will be discontinued at the end of 2026.

Please note that this product will be discontinued at the end of 2026.The pASG-IBA105 vector is designed for expression of recombinant proteins with an N-terminal Twin-Strep-tag® in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Twin-Strep-tag®
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: E. coli
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in E. coli
Promoter: tet Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 3952 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

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