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pESG-IBAwt1 vector

Mammalian expression vector without affinity tag


Content: 1 Stk
The pESG-IBAwt1 vector is designed for high-level, stable, and non-replicative transient expression of target proteins without affinity tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.


Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Mammalian cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in mammalian cells
Promoter: CMV Promoter
Resistance: Ampicillin, Neomycin
Sequence: See Documents
Size: 6061 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature


Competent E. coli TOP10
Chemically competent cells for plasmid propagation
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Entry vector for generation of StarGate donor vectors

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