Strep-Tactin® AP
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2-1503-001
Strep-Tactin® AP
Strep-Tactin® AP conjugate for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins
Content
For the specific detection of Strep-tag® and Twin-Strep-tag® fusion proteins, Strep-Tactin® is available conjugated to alkaline phosphatase (AP). The proteins can be captured on a membrane or microplate. After application of Strep-Tactin® AP, they can be visualized in a chromogenic reaction.
Strep-Tactin® AP cross-reacts with biotin and biotinylated proteins (e.g. the biotin carboxyl carrier protein in E. coli). To prevent the detection of false positives, prepare biotin blocking buffer (2.5 mg/ml avidin in PBS) and use for biotin masking according to our protocol.
| Amount: | 500 µl |
|---|---|
| Form: | Suspension in PBS |
| Possible Application: | Western blot, detection in microplates |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
GFP-Strep-tagII control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
Strep-tag®II peptide
Synthetic peptide recognized by Strep-Tactin® and Strep-Tactin®XT
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