Strep-Tactin® AP conjugate
Strep-Tactin® AP conjugate for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins
The streptavidin variant Strep-Tactin® is available as conjugate with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. The AP conjugate is suitable for chromogenic reaction.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® AP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.
|Form:||Suspension in PBS|
|Possible Application:||Western blot, detection in microplates|
|Specificity:||Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin|
|Stability:||12 months after shipping|