6-6310-300

Strep-Tactin® TACS Agarose Column

Gravity flow column suitable for traceless affinity cell and exosome isolation

€37.00*

Content
Strep-Tactin® TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The cells are eluted and ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin® TACS Agarose columns also suitable for negative cell selection or depletion approaches.

Specifications

Form: Pre-packed in buffer
Possible Application: Affinity chromatographic cell or exosome isolation
Support: Crosslinked agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

TACS Column Adapter
Adapter for Strep-Tactin® TACS Agarose columns that fits onto standard tubes
Flow Restrictor, pack of 5
Flow restrictor for 1 ml Strep-Tactin® TACS Agarose Column
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
10x Buffer CI
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA

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50% suspension for traceless affinity cell and exosome isolation
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