6-6310-001

Strep-Tactin® TACS Agarose Column

Gravity flow column suitable for traceless affinity cell and exosome isolation
Quantity Unit price
1-4
€75.00*
5-9
€63.00*
From 10
€56.00*
Content
Strep-Tactin® TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The cells are eluted and ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin® TACS Agarose columns also suitable for negative cell selection or depletion approaches.

Specifications

Form: Pre-packed in buffer
Possible Application: Affinity chromatographic cell or exosome isolation
Support: Crosslinked agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

TACS Column Adapter
Adapter for Strep-Tactin® TACS Agarose columns that fits onto standard tubes
Flow Restrictor, pack of 5
Flow restrictor for 1 ml Strep-Tactin® TACS Agarose Column
10x Buffer CI
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA

Related Products

Strep-Tactin® TACS Agarose
50% suspension for traceless affinity cell and exosome isolation
Strep-Tactin® Magnetic Microbeads
Strep-Tactin® multimers conjugated with magnetic microbeads
Strep-Tactin® PE
Fluorophore-conjugated Strep-Tactin® for cell staining
Strep-Tactin® APC
Fluorophore-conjugated Strep-Tactin® for cell staining