6-6310-001
Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for cell and exosome isolation via affinity chromatography
Strep-Tactin® TACS Agarose columns are ready-to-use columns for cell selection or depletion via affinity chromatography. They contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the optimal use for cell-related approaches.
The columns can be loaded with a protein of choice that is fused to a Twin-Strep-tag®. This can be for example an antibody or an antigen, which captures the cells inside the columns. Biotin addition causes the dissociation of strep-tagged proteins from Strep-Tactin®, leading to the elution of cells.
Alternatively, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated proteins (irreversible binding) for negative cell selection or depletion.
Cell Binding Capacity: | 2 x 10^7 (0.3 ml) or 1 x 10^8 (1 ml) target cells |
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Form: | Pre-packed in buffer |
Possible Application: | Cell isolation or depletion via affinity chromatography |
Support: | Crosslinked agarose |
Storage: | 2-8 °C |
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Stability: | 6 months after shipping |
Shipping: | Room temperature |