Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
Strep-Tactin® TACS Agarose columns are ready-to-use columns for cell selection or depletion via affinity chromatography. They contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the optimal use for cell-related approaches.
The columns can be loaded with a protein of choice that is fused to a Twin-Strep-tag®. This can be for example an antibody or an antigen, which captures the cells inside the columns. Biotin addition causes the dissociation of strep-tagged proteins from Strep-Tactin®, leading to the elution of cells.
Alternatively, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated proteins (irreversible binding) for negative cell selection or depletion.
|Cell Binding Capacity:||2 x 10^7 (0.3 ml) or 1 x 10^8 (1 ml) target cells|
|Form:||Pre-packed in buffer|
|Possible Application:||Cell isolation or depletion via affinity chromatography|
|Stability:||6 months after shipping|
Example protocol: Isolation of human CD3+ T cells from PBMCs, whole blood or buffy coat (1 ml column)
Example protocol: Isolation of murine CD4+ T cells from splenocytes (0.3 ml column)
Protocol for Fab-TACS® affinity chromatographic exosome isolation (1 ml column)
MSDS Strep-Tactin® TACS Agarose
Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Enable a Stable Non-Spilling Loading of T Cells and Their Magnetic Accumulation (Boosz, P. et al., 2021, Cancers)
5-Aza-2-deoxycytidine alleviates the progression of primary biliary cholangitis by suppressing the FoxP3 methylation and promoting the Treg/ Th17 balance (Jiang, T. et al., 2021, International Immunopharmacology)
Loading of Primary Human T Lymphocytes with Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Does Not Impair Their Activation after Polyclonal Stimulation. (Mühlberger, M., et al., 2020, Cells)