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2-1042-025

Buffer BXT (10x)

Strep-Tactin®XT elution buffer

€49.00*

Content
Buffer BXT (10x) is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with very high affinity, but reversibly, and thereby allows the efficient elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the resin. Please note that Buffer BXT (10x) is not applicable for elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from Strep-Tactin®, since biotin irreversibly binds to these resins, and thus Strep-Tactin® cannot be regenerated.

Specifications

Concentration: 10x concentrated
Form: Solution
Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

MagStrep "type3" XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer