Buffer BXT (10x)
Strep-Tactin®XT elution buffer
10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer. It has to be diluted before use. Biotin reversibly binds to Strep-Tactin®XT, which allows the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins.
Please note that biotin binds irreversibly to Strep-Tactin®. Therefore, 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® proteins from Strep-Tactin® resins, since they cannot be regenerated after use.
|Possible Application:||Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins|
|Stability:||12 months after shipping|