2-1016-002

Biotin

Biotin powder for preparation of Strep-Tactin®XT elution buffer

€66.00*

Content: 1 Stk
Content
Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to the biotin-binding pockets of streptavidin, Strep-Tactin®, and Strep-Tactin®XT with different affinities. As a result, it allows the efficient elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from Strep-Tactin®XT, while maintaining the reusability of the resin. It can be used for preparation of Strep-Tactin®XT elution buffer, Buffer BXT. In addition, biotin can be used for elution of biotinylated proteins from Strep-Tactin®, where it binds irreversibly to the resin. Hence, Strep-Tactin® cannot be regenerated in this kind of application.

Specifications

Alternative Names: Biotin (vitamin B7, vitamin H, coenzyme R)
Form: Powder
Molecular Weight: 244.3 g/mol
Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature
Manual Strep-Tactin®XT purification
Short protocol Strep-Tactin®XT purification
Short protocol Strep-Tactin®XT high capacity purification

Accessories

MagStrep "type3" XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set