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2-5031-005

Strep-Tactin®XT 4Flow® high capacity column

Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

€256.00*

Content

Strep-Tactin®XT 4Flow® high capacity columns are applicable to the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. It is recommended for proteins of all classes, especially low abundant, large, and highly expressed proteins, due to its pH compatibility, target purity (> 95%), and high binding affinity. Multimeric protein complexes might bind too strongly to our Strep-Tactin®XT 4Flow® high capacity resins, preventing efficient elution. Strep-Tactin® resins are recommended for these proteins.
With Strep-Tactin®XT 4Flow® high capacity, denaturing agents such as urea can be used. In comparison to Strep-Tactin®XT 4Flow®, Strep-Tactin®XT 4Flow® high capacity has a higher density of immobilized Strep-Tactin®XT, which leads to a higher yield of purified protein.
Purify large samples faster with our WET FRED. 

Check out our resin specification overview for details about recommended applications.

Specifications

Bead Size: 50 - 150 µm spherical
Dynamic Protein Binding Capacity: 14 mg/ml resin
Form: Pre-packed gravity flow column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

WET FRED Set
Application aid for large volumes of lysates or culture supernatants
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

Related Products

Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity
MagStrep® Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® FPLC column
FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins