2-1042-025
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Buffer BXT (10x) is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with very high affinity, but reversibly, and thereby allows the efficient elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the resin.
Please note that Buffer BXT (10x) is not applicable for elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from Strep-Tactin®, since biotin irreversibly binds to these resins, and thus Strep-Tactin® cannot be regenerated.
Concentration: | 10x concentrated |
---|---|
Form: | Solution |
Possible Application: | Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins |
Storage: | 2-8 °C |
---|---|
Stability: | 12 months after shipping |
Shipping: | Room temperature |