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Detection of Strep-tag® fusion proteins

Two different detection systems are provided:

  1. detection via Strep-Tactin® conjugates (for Strep-/Twin-Strep-tag® proteins)
  2. detection via monoclonal antibodies against Strep-/Twin-Strep-tag® 

Read which detection system is suitable for your applications:

Western blot

For direct detection of Strep-tag® fusion proteins in Western blots use:

ELISA

For protein detection in ELISA use:

Strep-Tactin Strep-Tactin®XT StrepMAB-Classic StrepMAB-Immo
AP DY-488 DY-488 DY-488
HRP DY-549 DY-549 DY-549
unconjugated DY-649 DY-649 DY-649
    HRP unconjugated
    unconjugated  

For nearly irreversible binding of StrepMAB-Immo, the Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein).

Immunocytochemistry/Immunohistochemistry

Visualize your Strep-tag® protein Strep-Tactin®XT, StrepMAB-Classic, or StrepMAB-Immo fluorescent conjugates. They are available with:

Strep-Tactin®XT
StrepMAB-Classic
StrepMAB-Immo

For nearly irreversible binding of StrepMAB-Immo, the Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein).

FACS

For staining (Twin-) Strep-tagged proteins in FACS we recommend to use:

Strep-Tactin® Strep-Tactin®XT StrepMAB-Immo
Strep-Tactin® PE  DY-488 DY-488
Strep-Tactin® APC DY-549 DY-549
  DY-649 DY-649

For nearly irreversible binding of StrepMAB-Immo, the Strep-tag® must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein).