Protein detection

Two different detection systems are provided:

  1. detection via Strep-Tactin® conjugates (for Strep-/Twin-Strep-tag® proteins)
  2. detection via monoclonal antibodies against Strep-/Twin-Strep-tag® or His-tag

Read which detection system is suitable for your applications:

Western blot

For direct detection of Strep-tag® fusion proteins in Western blots

  • via chromogenic and/or chemiluminescence reaction (no secondary antibody needed!) use
    - Strep-Tactin® HRP conjugate (HRP, horseradish peroxidase)
    - StrepMAB-Classic HRP conjugate
  • via chromogenic reaction
    - Strep-Tactin® AP conjugate (AP, alkaline phosphatase)
ELISA

For protein detection in ELISA use

  • StrepMAB-Classic, unconjugated
  • Rabbit anti Mouse pAb, HRP conjugated (secondary antibody)
  • Strep-Tactin® HRP conjugate
FACS/Immunocytochemistry/Immunohistochemistry

Visualize your Strep-tag® protein with the following dyes:

Strep-Tactin® , StrepMAB-Classic and StrepMAB-Immo are available with single fluorescent conjugates at different  wavelengths (488 nm, 546 nm, 645 nm):

  • Strep-tag® fluorescent Chromeo 488 conjugates of Strep-Tactin®, StrepMAB Classic and StrepMAB Immo (50 µg each)
  • Strep-tag® fluorescent Chromeo 546 conjugates of Strep-Tactin®, StrepMAB Classic and StrepMAB Immo (50 µg each)
  • Strep-tag® fluorescent Oyster 645 conjugates of Strep-Tactin®, StrepMAB Classic and StrepMAB Immo (50 µg each)