MagStrep Strep-Tactin®XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
MagStrep Strep-Tactin®XT beads can be used for the purification of strep-tagged fusion proteins in batch in single or high-throughput format.
For cell purification we offer specific magnetic beads with a smaller diameter (1µm) and for biotinylated proteins we recommend our MagStrep “type3” Strep-Tactin® beads.
MagStrep Strep-Tactin®XT beads show low non-specific protein binding due to their agarose surface and high specificity of Strep-Tactin®XT towards Strep-tag. This results in outstanding target protein purity and makes the MagStrep Strep-Tactin®XT beads a perfect tool for the isolation of proteins or the identification of complexes via pull-downs, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP). MagStrep Strep-Tactin®XT beads are suitable for fast small-scale purifications in reaction tubes as well as 96-well plates.
The beads can be easily and swiftly separated with a magnetic separator. This eliminates the need for centrifugation and reduces the risk of disturbing the pellet or loss of beads within the wash step to zero. The elution of target proteins or complexes can be triggered under mild conditions with the biotin-containing Buffer BXT or under denaturing conditions by boiling in SDS gel loading buffer.
If you want to learn more about how to improve your yield when working with magnetic beads, please check out our guide on the optimiziation of magnetic bead-based protein purification.
|Bead Size:||30 µm (average)|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Strep-tag®II and Twin-Strep-tag®|
|Support:||6% agarose, crosslinked, spherical magnetic beads|
|Twin-Strep-tag® Binding Capacity:||33 mg/ml|
|Stability:||12 months after shipping|