5-4005-001

pASG-IBA5 vector

Cytoplasmic E. coli expression vector encoding an N-terminal Strep-tag®II

€219.00*

Content
The pASG-IBA5 vector is designed for expression of recombinant proteins with an N-terminal Strep-tag®II in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Strep-tag®II
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: E. coli
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in E. coli
Promoter: tet Promoter
Resistance: Ampicillin
Sequence: See Documents
Size: 3892 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Anhydrotetracycline
Derivative of tetracycline for induction of tetracycline-controlled promoters
Competent E. coli TOP10
Chemically competent cells for plasmid propagation
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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