2-1324-000

pASK-IBA5C vector

Cytoplasmic E. coli expression vector encoding an N-terminal Strep-tag®II and chloramphenicol resistance

€183.00*

Content
pASK-IBA5C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), and the inducible tetracycline promoter/operator for the regulated expression of proteins.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. The recombinant protein will be localized in the cytoplasm.

Specifications

Affinity Tag N'-terminal: Strep-tag®II
Cloning Method: Direct cloning using e.g. restriction enzyme BsaI in MCS
Concentration: 250 ng/µl
Expression Host: E. coli
Form: Suspension in TE buffer
Possible Application: Expression plasmid for Strep-tag®II fusion proteins
Promoter: tet Promoter
Resistance: Chloramphenicol
Sequence: See Documents
Size: 3014 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Anhydrotetracycline
Derivative of tetracycline for induction of tetracycline-controlled promoters
Competent E. coli TOP10
Chemically competent cells for plasmid propagation

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