5-5104-001

pCSG-IBA104 vector

Mammalian expression vector for secretion of N-terminal Twin-Strep-tag® fusion proteins

€219.00*

Content
pCSG-IBA104 is a large expression vector with universal features for transient expression of target proteins with an N-terminal Twin-Strep-tag® as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells. The expressed protein is transferred into the medium due to the BM40 secretory signal peptide. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Twin-Strep-tag®
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Mammalian cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in mammalian cells
Promoter: CMV Promoter
Resistance: Ampicillin, Neomycin
Sequence: See Documents
Size: 11022 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Competent E. coli TOP10
Chemically competent cells for plasmid propagation
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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