5-4504-001

pESG-IBA104 vector

Mammalian expression vector for secretion of N-terminal Twin-Strep-tag® fusion proteins

€219.00*

Content
The pESG-IBA104 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with an N-terminal Twin-Strep-tag® in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). BM40 secretory signal peptide is encoded for the transfer of the expressed protein into the medium. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

Affinity Tag N'-terminal: Twin-Strep-tag®
Cloning Method: Direct cloning using restriction enzyme Esp3I
Concentration: 250 ng/µl
Expression Host: Mammalian cells
Form: Suspension in TE buffer
Possible Application: Vector for recombinant expression in mammalian cells
Promoter: CMV Promoter
Resistance: Ampicillin, Neomycin
Sequence: See Documents
Size: 6217 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Competent E. coli TOP10
Chemically competent cells for plasmid propagation
pENTRY-IBA51 vector
Entry vector for generation of StarGate donor vectors

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