Benefits of Strep-tag® vs His-tag purification
Strep-tag® and His-tag are both widely used tools for affinity purification via a peptide tag. But when it comes to purity and versatility in applications, some differences between both systems are obvious.
Pros and Cons of Strep-tag® and His-tag
+ high purity, even under denaturing conditions and in batch purification
+ resin can be recharged
|Cons||- less yield compared to His-tag
||- not suitable for metal proteins
- interacts unspecifically with complex forming amino acids
- does not tolerate chelating agents
- works only best for E. coli expression (often problematic after mammalia expression)
- time consuming elution gradient for higher purity
Watch this webinar and learn more about the benefits and drawbacks of the two systems:Strep-tag® vs His-tag
The 3rd generation Strep-tag® system can now be used for purification under denaturing conditions using up to 6 M urea. Check the application note for a comparison of Strep-tag® system III to His-tag:NiNTA purification under denaturing conditions.
Download experimental data:
IBA is the original manufacturer of the Strep-tag® system and provides a complete portfolio around protein purification using this affinity system!
High affinity meets high capacity: