Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution. Desthiobiotin powder can be used for preparation of Strep-Tactin® elution buffer, Buffer E. Please note that desthiobiotin is not applicable for elution from Strep-Tactin®XT resins.
|Alternative Names:||5-Methyl-2-oxo-4-imidazolidinehexanoic acid|
|Molecular Weight:||214.3 g/mol|
|Possible Application:||Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins|
|Stability:||12 months after shipping|
Manual Strep-Tactin® purification
Short protocol Strep-Tactin® purification