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Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
Desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution. Desthiobiotin powder can be used for preparation of Strep-Tactin® elution buffer, Buffer E.
Please note that desthiobiotin is not applicable for elution from Strep-Tactin®XT resins.
| Alternative Names: | 5-Methyl-2-oxo-4-imidazolidinehexanoic acid |
|---|---|
| Form: | Powder |
| Molecular Weight: | 214.3 g/mol |
| Possible Application: | Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Strep-Tactin® Sepharose® resin
Content:
4 ml
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Content:
1 ml
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
Content:
4 ml
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Content:
1 ml
Strep-Tactin® Superflow® high capacity column
Content:
1 ml
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity FPLC column
Content:
1 ml
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
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