6-5510-050

Strep-Tactin® Magnetic Microbeads

Strep-Tactin® multimers conjugated with magnetic microbeads

€205.00*

Content
Strep-Tactin® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin®. They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.

Specifications

Bead Size: 1 - 3 µm
Form: Suspension in DPBS
Possible Application: Cell isolation (magnetic)
Recommended Usage: 15 µl per 1 x 10^7 total cells

Shipping information

Storage: 2-8 °C; Do not freeze!
Stability: 6 months after shipping
Shipping: Blue ice

Accessories

StrepMan Magnet
Permanent magnet for cell isolations using Strep-Tactin® Magnetic Microbeads
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
10x Buffer CI
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA
MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
MHC I-Strep HLA-A*0201; SARS-CoV-2 (VLNDILSRL)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide

Related Products

Strep-Tactin® PE or APC
Fluorophore-conjugated Strep-Tactin® for cell staining
Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
Strep-Tactin® TACS Agarose
50% suspension for traceless affinity cell and exosome isolation