6-5510-050
Strep-Tactin® Magnetic Microbeads
Strep-Tactin® multimers conjugated with magnetic microbeads
| Quantity | Unit price |
|---|---|
| 1-2 |
€226.00*
|
| 3-9 |
€215.00*
|
| From 10 |
€192.00*
|
Content
Please note: Strep-Tactin® Magnetic Microbeads were specifically developed for cell isolation. For protein purification, we offer MagStrep Strep-Tactin®XT beads and MagStrep "type3" Strep-Tactin® beads, which have an agarose coating that prevents aggregation and unspecific binding as well as ensures a high protein binding capacity.
Strep-Tactin® Magnetic Microbeads are suitable for multimerizing Strep-Tag®II- or Twin-Strep-tag®-fusion proteins for subsequent cell selection. Alternatively, cells that express a strep-tagged surface protein can be isolated.
Example applications are the isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to the microbeads.
Biotin reverses the binding of the microbeads to strep-tagged proteins in all isolation approaches. This way, the microbeads can be easily removed from the cells, preventing adverse effects on further downstream applications.
Strep-Tactin® Magnetic Microbeads are suitable for multimerizing Strep-Tag®II- or Twin-Strep-tag®-fusion proteins for subsequent cell selection. Alternatively, cells that express a strep-tagged surface protein can be isolated.
Example applications are the isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to the microbeads.
Biotin reverses the binding of the microbeads to strep-tagged proteins in all isolation approaches. This way, the microbeads can be easily removed from the cells, preventing adverse effects on further downstream applications.
| Bead Size: | 1 - 3 µm |
|---|---|
| Form: | Suspension in DPBS |
| Possible Application: | Cell isolation (magnetic) |
| Recommended Usage: | 15 µl per 1 x 10^7 total cells |
| Storage: | 2-8 °C; Do not freeze! |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Protective T cell receptor identification for orthotopic reprogramming of immunity in refractory virus infections (Stief et al., 2022, Molecular Therapy)
Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments (Nannini et al., 2021, Monoclonal Antibodies)
Rab1-AMPylation by Legionella DrrA is allosterically activated by Rab1 (Du et al., 2021, Nature Communications)
Accessories
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
CD3 Fab-Strep, human
Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD3
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Gravity flow column suitable for traceless affinity cell and exosome isolation
