6-5510-050
Strep-Tactin® Magnetic Microbeads
Strep-Tactin® multimers conjugated with magnetic microbeads
| Quantity | Unit price |
|---|---|
| 1-2 |
€205.00*
|
| 3-9 |
€195.00*
|
| From 10 |
€174.00*
|
Content
Strep-Tactin® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin®. They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®.
Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.
| Bead Size: | 1 - 3 µm |
|---|---|
| Form: | Suspension in DPBS |
| Possible Application: | Cell isolation (magnetic) |
| Recommended Usage: | 15 µl per 1 x 10^7 total cells |
| Storage: | 2-8 °C; Do not freeze! |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
MHC I-Strep HLA-A*0201; SARS-CoV-2 (VLNDILSRL)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
Related Products
Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
