Staining and Detection
Immunostaining for protein detection
The term immunostaining covers a variety of methods for the specific detection and quantification of proteins from diverse samples, whereby “immuno” refers to the general use of antibodies. Immunostaining techniques include immunohisto- and immunocytochemistry flow cytometry, enzyme-linked immunosorbent assays (ELISA) and western blots.
These different methods are based on either colorimetric, chemiluminescence or fluorescence signals for detection. Besides the signal, the type of detection varies according to the experimental procedure. It can be chosen between direct detection, where a primary antibody carrying a label is used, and an indirect detection using an unlabeled primary combined with a labeled secondary antibody. As convenient alternative, we offer several solutions based on the Strep-tag® technology using Strep-Tactin® or Strep-Tactin®XT combined with Strep-tag®II or Twin-Strep-tag®.