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Mammalian expression vectors

 

pDSG-IBA vectors

pDSG-IBA is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system. Efficient extrachromosomal replication due to EBV oriP driven by EBNA-1. Requires MEXi 293E cell line or any other cell line expressing EBNA-1 gene.

  • CMV promoter: high level constitutive expression

  • oriP: origin of replication from Epstein-Barr Virus for extrachromosomal replication driven by EBNA-1 (MEXi 293E cells express EBNA-1)

  • ColE1 + Amp: for propagation in E. coli

  • BM40: secretion signal to transfer the expressed protein into the medium (not on all vectors available)

 

pESG-IBA vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori. NeoR for generation of stable cells lines.

  • CMV promoter: high level constitutive expression

  • SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)

  • ColE1 + Amp: for propagation in E. coli

  • NeoR: neomycin resistance gene to generate stable cell lines

  • BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

pCSG-IBA vectors

pCSG-IBA is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). Extrachromosomal replication by either the more powerful oriP (cell line independent due to EBNA-1 gene provided by vector ) or SV40 ori (cell line dependent) possible.

  • CMV promoter: high level constitutive expression

  • oriP: origin of replication from Epstein Barr Virus for extrachromosomal replication driven by EBNA-1

  • EBNA-1 gene: Expression of EBNA-1 for initiation of extrachromosomal replication by oriP

  • SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)

  • NeoR: neomycin resistance gene to generate stable cell lines

  • ColE1 + Amp: for propagation in E. coli

  • BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

 

 

Mammalia expression vectors are available with multiple tags:

Host Promoter Tags
Mammalia CMV Strep-tag®II,
Twin-Strep-tag®,
6xHis-tag,
FLAG-tag,
GST-tag with PreScission™ (PSC) site
Mammalian Expression Vectors Overview

Mammalia expression vectors

 

Expression vector product list for mammalia

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
Forw sequencing primer for pESG-, pCSG-, pDSG- and pEXPR-IBA vectors (HPLC-purified) 1 nmol 5-0000-121 USD 26.00  
Rev sequencing primer for pESG-, pCSG-, pDSG- and pEXPR-IBA vectors (HPLC-purified) 1 nmol 5-0000-122 USD 26.00  
 
Forward and Reverse sequencing primers for pESG-, pCSG-, pDSG- and pEXPR-IBA vectors (HPLC-purified) 1 nmol each 5-0000-124 USD 34.00  
 
Competent cells E. coli TOP10 20 rxns 5-1600-020 USD 108.00  

One-shot chemically competent E.coli TOP10 cells, 20reactions. ...

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StarGate® Acceptor Vector pESG-IBAwt1 5µg 5-4400-001 USD 260.00  
pESG

The pESG-IBAwt1 vector allows the high-level stable and non-replicative transient expression of control proteins without tag in most mammalian hosts. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells as well as the Ampicillin resistance cassette for selection in E.coli and the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the ...

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StarGate® Acceptor Vector pESG-IBAwt2 5µg 5-4401-001 USD 260.00  
pESG

The pESG-IBAwt2 vector allows the high-level stable and non-replicative transient expression of control proteins without tag in most mammalian hosts. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells, the BM40 signal for secretion of the recombinant protein as well as the Ampicillin resistance cassette for selection in E.coli and the Neomycin resistance gene for selection of stable cell lines. The ...

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StarGate® Acceptor Vector pESG-IBA3 5µg 5-4403-001 USD 260.00  
pESG

The pESG-IBA3 vector allows the expression of Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the Strep-tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

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StarGate® Acceptor Vector pESG-IBA5 5µg 5-4405-001 USD 260.00  
pESG

The pESG-IBA5 vector allows the expression of Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the Strep-tag® for N-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

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StarGate® Acceptor Vector pESG-IBA23 5µg 5-4423-001 USD 260.00  
pESG

The pESG-IBA23 vector allows the expression of GST/Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the GST-tag for N-terminal and the Strep -tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm. The additional PreScission protease cleavage ...

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StarGate® Acceptor Vector pESG-IBA25 5µg 5-4425-001 USD 260.00  
pESG

The pESG-IBA25 vector allows the expression of GST-tag-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the GST-tag for N-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm. The additional PreScission protease cleavage site allows the removal of the GST-tag.

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StarGate® Acceptor Vector pESG-IBA33 5µg 5-4433-001 USD 260.00  
pESG

The pESG-IBA33 vector allows the expression of 6xHistidine-tag-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the 6xHistidine -tag for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

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StarGate® Acceptor Vector pESG-IBA35 5µg 5-4435-001 USD 260.00  
pESG

The pESG-IBA35 vector allows the expression of 6xHistidine-tag-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the 6xHistidine-tag for N-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

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