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Mammalia expression vectors

Mammalia expression vectors are available with a multitude of different features:

Host Promoter Tags
Mammalia CMV Strep-tag®II,
Twin-Strep-tag®,
6xHis-tag,
FLAG-tag,
GST-tag with PreScission™ (PSC) site

Further special vectors with protease cleavage sites are available for classic cloning and the respective expression vectors. Read more below:

Detailed information about mammalia espression vectors

StarGate Acceptor Vectors for Mammalia

pDSG-IBA vectors

  • Optimized for MEXi, Mammalian Expression IBA, and are also suitable for other mammalian cell cultures
  • High level constitutive expression in mammalian cells by the CMV promoter
  • Extrachromosomal replication due to Epstein Barr Virus replication origin (oriP) - requires chromosomal expression of the EBNA-1 gene, e.g. like in MEXi 293E cells
  • ColEl ori and ampicillin resistance gene support propagation in E. coli
  • BM40 option for secretion of protein into the medium

pESG-IBA vectors

  • High-level constitutive expression in mammalian cells by the CMV promoter
  • Neomycin resistance for generation of stable cell lines
  • ColEl ori and ampicillin resistance gene support propagation in E. coli
  • BM40 option for secretion of protein into the medium

pCSG-IBA vectors

  • High-level constitutive expression in mammalian cells by the CMV promoter
  • extrachromosomal replication due to Epstein Barr Virus replication origin (oriP) and EBNA-1
  • prolonged expression of the inserted GOI under G418 selection without the need for making stable cell lines
  • ColEl ori and ampicillin resistance gene support propagation in E. coli
  • BM40 option for secretion of protein into the medium
Classic cloning vectors for mammalia
pEXPR-IBA vectors from the Classic Cloning series for expression of Strep-tagged proteins in mammalian cells are available with different protease cleavage sites.
The human cytomegalovirus (CMV) immediate early promoter provides strong expression in a wide range of mammalian cells. To prolong expression in transfected cells, the vector will replicate in cell lines that are latently infected with SV40 large T-antigen (e.g. COS7). In addition, the Neomycin resistance gene allows direct selection of stable cell lines.

 

pEXPR-IBA Features Benefits
Strep-tag®II Purification of recombinant protein using Strep-Tactin® matrices
CMV immediate-early promoter/enhancer High-level expression in a wide range of mammalian cells
Multiple cloning site Insertion of gene of interest and fusion to Strep-tag®. Compatible with pASK-IBA vectors
Neomycin resistance gene Selection of stable transfectants in mammalian cells
Ampicillin resistance gene Selection in E. coli
pUC origin High-copy number replication in E. coli
Factor Xa, thrombin (thb),
enterokinase (ent) and
TEV cleavage sites
Removal of the Strep-tag® if required (generally not necessary)

Mammalia expression vector overview

List of mammalia expression vectors and their features

 

Expression vector product list for mammalia

Please use filters "Category" and "Subcategory" to quickly select your product from the list below:


Product Contents Cat.no Price   Order
3 pEXPR-IBA vectors of choice 5 µg each 2-1900-000 USD 521.00  
 
pEXPR-IBA-Vectors798px

Special offer: 3 pEXPR-IBA vectors of choice for the price of two! ...

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pEXPR-IBA7 5 µg 2-1907-000 USD 260.00  
2-1907-000

The pEXPR-IBA7 vector allows the expression of Strep-tag®-fusion-proteins in mammalian cells. ...

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pEXPR-IBA13 5 µg 2-1913-000 USD 260.00  
 
2-1913-000

The pEXPR-IBA13 vector allows the expression of Strep-tag®-fusion-proteins in mammalian cells. ...

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pEXPR-IBA15 5 µg 2-1915-000 USD 260.00  
2-1915-000

The pEXPR-IBA15 vector allows the expression of Strep-tag®-fusion-proteins in mammalian cells. ...

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pEXPR-IBA17 5 µg 2-1917-000 USD 260.00  
2-1917-000

The pEXPR-IBA17 vector allows the expression of Strep-tag®-fusion-proteins in mammalian cells. ...

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Forw sequencing primer for pESG-, pCSG-, pDSG- and pEXPR-IBA vectors (HPLC-purified) 1 nmol 5-0000-121 USD 26.00  
Rev sequencing primer for pESG-, pCSG-, pDSG- and pEXPR-IBA vectors (HPLC-purified) 1 nmol 5-0000-122 USD 26.00  
Forward and Reverse sequencing primers for pESG-, pCSG-, pDSG- and pEXPR-IBA vectors (HPLC-purified) 1 nmol each 5-0000-124 USD 34.00  
Competent cells E. coli TOP10 20 rxns 5-1600-020 USD 108.00  

One-shot chemically competent E.coli TOP10 cells, 20reactions. ...

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StarGate® Acceptor Vector pESG-IBAwt1 5µg 5-4400-001 USD 260.00  
pESG

The pESG-IBAwt1 vector allows the high-level stable and non-replicative transient expression of control proteins without tag in most mammalian hosts. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells as well as the Ampicillin resistance cassette for selection in E.coli and the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the ...

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StarGate® Acceptor Vector pESG-IBAwt2 5µg 5-4401-001 USD 260.00  
pESG

The pESG-IBAwt2 vector allows the high-level stable and non-replicative transient expression of control proteins without tag in most mammalian hosts. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells, the BM40 signal for secretion of the recombinant protein as well as the Ampicillin resistance cassette for selection in E.coli and the Neomycin resistance gene for selection of stable cell lines. The ...

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StarGate® Acceptor Vector pESG-IBA3 5µg 5-4403-001 USD 260.00  
pESG

The pESG-IBA3 vector allows the expression of Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the Strep-tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.

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