Streptavidin - Immobilization of biotin or biotinylated proteins
Streptavidin, lyophilized
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket, as compared to native streptavidin.The streptavidin:biotin system is widely used tool for immobilization and assays of biotinylated proteins or other biotin conjugated molecules.
Not suitable for Strep-tag® applications!
Streptavidin Products
| Product | Contents | Cat.no | Price | Order | |||
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| Streptavidin lyophilized | 1 mg | 2-0203-001 | USD 55.00 | ||||
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Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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| Streptavidin lyophilized | 10 mg | 2-0203-010 | USD 273.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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| Streptavidin lyophilized | 100mg | 2-0203-100 | USD 410.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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Streptavidin - Product Specifications
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Form
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Lyophilized in 10 mM potassium phosphate buffer pH 6.5
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MW per tetramer
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~53 000
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Extinction coefficient per subunit
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ε280 = 41326 M-1 cm-1
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Specific activity
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> 17 U/mg (one unit binds 1 µg D-biotin)
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Purity
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> 95 % as estimated by SDS-PAGE
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Proteolytic activity
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< 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)
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Reconstitution
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Dissolve with water
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Storage (lyophilized)
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-20 °C
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Stability (lyophilized)
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2 years after shipping
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Storage (dissolved)
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4 °C; for longer storage in dissolved form add 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter
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Shipment
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Room temperature
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