Streptavidin - Immobilization of biotin or biotinylated proteins
Streptavidin, lyophilized
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket, as compared to native streptavidin.The streptavidin:biotin system is widely used tool for immobilization and assays of biotinylated proteins or other biotin conjugated molecules.
Not suitable for Strep-tag® applications!
Streptavidin Products
| Product | Contents | Cat.no | Price | Order | |||
|---|---|---|---|---|---|---|---|
| Streptavidin lyophilized | 1 mg | 2-0203-001 | USD 55.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
|||||||
| Streptavidin lyophilized | 10 mg | 2-0203-010 | USD 273.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
|||||||
| Streptavidin lyophilized | 100mg | 2-0203-100 | USD 410.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
|||||||
Streptavidin - Product Specifications
|
Form
|
Lyophilized in 10 mM potassium phosphate buffer pH 6.5
|
|
MW per tetramer
|
~53 000
|
|
Extinction coefficient per subunit
|
ε280 = 41,326 M-1 cm-1
|
|
Specific activity
|
> 17 U/mg (one unit binds 1 µg D-biotin)
|
|
Purity
|
> 95 % as estimated by SDS-PAGE
|
|
Reconstitution
|
Dissolve with water. Any occurring turbidities are not a quality defect.
To improve the solubility of streptavidin use 50mM NaCl. |
|
Storage (lyophilized)
|
-20 °C
|
|
Stability (lyophilized)
|
2 years after shipping
|
|
Storage (dissolved)
|
4 °C; for longer storage in dissolved form add 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter
|
|
Shipment
|
Room temperature
|
