Streptavidin - Immobilization of biotin or biotinylated proteins
Streptavidin, lyophilized
The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules, like proteins and nucleic acids. IBA provides bulk amounts of lyophilized streptavidin with competitive prices in reliable high quality to generate
• streptavidin coated surfaces (microplates, SPR-chips or beads)
• detection reagents conjugated with e.g. fluorescent dyes
Streptavidin Products
| Product | Contents | Cat.no | Price | Order | |||
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| Streptavidin | 1 mg | 2-0203-001 | USD 59.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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| Streptavidin | 10 mg | 2-0203-010 | USD 289.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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| Streptavidin | 100 mg | 2-0203-100 | USD 435.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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| Streptavidin | 1 g | 2-0203-101 | USD 2,183.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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| Streptavidin | 5 g | 2-0203-105 | USD 8,704.00 | ||||
 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ... |
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Streptavidin Specifications
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket, as compared to native streptavidin.
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Form
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lyophilized from a 25mg/ml solution in 10 mM potassium phosphate buffer pH 6.5
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MW per subunit
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13331 Da
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Extinction coefficient per subunit
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ε280 = 41,326 M-1 cm-1
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Specific activity
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> 17 U/mg (one unit binds 1 µg D-biotin)
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Purity
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> 95 % as estimated by SDS-PAGE
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Reconstitution
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dissolve with water (dissolved at 10 mg/ml, the solution will be buffered with 4.0 mM potassium phosphate pH 6.5). Any occurring turbidities are not a quality defect.
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Storage (lyophilized)
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recommended: -25°C to -15 °C
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Stability (lyophilized)
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24 month after shipping
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Storage (dissolved)
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recommended: 2 – 8 °C, for long term storage add 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
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Shipping
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room temperature
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