Home > Protein Production & Assays > Immobilization > Streptavidin - Biotin Immobilization & Assays

Streptavidin - Immobilization of biotin or biotinylated proteins

Streptavidin, lyophilized

The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules, like proteins and nucleic acids. IBA provides bulk amounts of lyophilized streptavidin with competitive prices in reliable high quality to generate
•    streptavidin coated surfaces (microplates, SPR-chips or beads)
•    detection reagents conjugated with e.g. fluorescent dyes

Streptavidin Products

Bulk order on request: strep-tag@iba-lifesciences.com
Product Contents Cat.no Price   Order
Streptavidin lyophilized 1 mg 2-0203-001 USD 57.00  
2-0203-001

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more
Streptavidin lyophilized 10 mg 2-0203-010 USD 281.00  
2-0203-010

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more
Streptavidin lyophilized 100 mg 2-0203-100 USD 422.00
2-0203-100

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more
Streptavidin lyophilized 1 g 2-0203-101 USD 2,119.00
streptavidin_lyophilized

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more
Streptavidin lyophilized 5 g 2-0203-105 USD 8,450.00
2-0203-105

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more

Streptavidin Specifications

Form
lyophilized from a 25mg/ml solution in 10 mM potassium phosphate buffer pH 6.5
MW per subunit
13331 Da
Extinction coefficient per subunit
ε280 = 41,326 M-1 cm-1
Specific activity
> 17 U/mg (one unit binds 1 µg D-biotin)
Purity
> 95 % as estimated by SDS-PAGE
Reconstitution
dissolve with water (dissolved at 10 mg/ml, the solution will be buffered with 4.0 mM potassium phosphate pH 6.5). Any occurring turbidities are not a quality defect.
Storage (lyophilized)
recommended: -25°C to -15 °C
Stability (lyophilized)
24 month after shipping
Storage (dissolved)
recommended: 2 – 8 °C, for long term storage add 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Shipping
room temperature

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket, as compared to native streptavidin.