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Streptavidin - Immobilization of biotin or biotinylated proteins

Streptavidin, lyophilized

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket, as compared to native streptavidin.The streptavidin:biotin system is widely used tool for immobilization and assays of biotinylated proteins or other biotin conjugated molecules.

Not suitable for Strep-tag® applications!

Streptavidin Products

Bulk order on request: strep-tag@iba-lifesciences.com
Product Contents Cat.no Price   Order
Streptavidin lyophilized 1 mg 2-0203-001 USD 55.00  

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more
Streptavidin lyophilized 10 mg 2-0203-010 USD 273.00  
2-0203-xxx

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more
Streptavidin lyophilized 100mg 2-0203-100 USD 410.00
2-0203-xxx

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a high degree of affinity and specificity with KD of ~1x10-15 M which makes it one of the strongest known non-covalent interaction. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogenity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native ...

Further details, manuals, data sheets, related products and more

Streptavidin - Product Specifications

Streptavidin Specifications
Form
Lyophilized in 10 mM potassium phosphate buffer pH 6.5
MW per tetramer
~53 000
Extinction coefficient per subunit
ε280 = 41326 M-1 cm-1
Specific activity
> 17 U/mg (one unit binds 1 µg D-biotin)
Purity
> 95 % as estimated by SDS-PAGE
Proteolytic activity
< 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)
Reconstitution
Dissolve with water
Storage (lyophilized)
-20 °C
Stability (lyophilized)
2 years after shipping
Storage (dissolved)
4 °C; for longer storage in dissolved form add 1 mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter
Shipment
Room temperature