2-1321-000

pASK-IBA2C vector

Periplasmic E. coli expression vector encoding a C-terminal Strep-tag®II and chloramphenicol resistance

€183.00*

Content
pASK-IBA2C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the C-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During secretion into the periplasmic space the ompA signal sequence will be cleaved off.

Specifications

Affinity Tag C'-terminal: Strep-tag®II
Cloning Method: Direct cloning using e.g. restriction enzyme BsaI in MCS
Concentration: 250 ng/µl
Expression Host: E. coli
Form: Suspension in TE buffer
Possible Application: Expression plasmid for Strep-tag®II fusion proteins
Promoter: tet Promoter
Resistance: Chloramphenicol
Sequence: See Documents
Size: 3061 bp

Shipping information

Storage: -20 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Anhydrotetracycline
Derivative of tetracycline for induction of tetracycline-controlled promoters
Competent E. coli TOP10
Chemically competent cells for plasmid propagation

Related Products

pASK-IBA4C vector
Periplasmic E. coli expression vector encoding an N-terminal Strep-tag®II and chloramphenicol resistance
pASK-IBA6C vector
Periplasmic E. coli expression vector encoding an N-terminal Strep-tag®II, chloramphenicol resistance and protease cleavage site
pASG-IBA2 vector
Periplasmic E. coli expression vector encoding a C-terminal Strep-tag®II
pASG-IBA4 vector
Periplasmic E. coli expression vector encoding an N-terminal Strep-tag®II