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2-1321-000
pASK-IBA2C vector
Please note that this product will be discontinued at the end of 2026.
Please note that this product will be discontinued at the end of 2026.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During secretion into the periplasmic space the ompA signal sequence will be cleaved off.
| Affinity Tag C'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using e.g. restriction enzyme BsaI in MCS |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Expression plasmid for Strep-tag®II fusion proteins |
| Promoter: | tet Promoter |
| Resistance: | Chloramphenicol |
| Sequence: | See Documents |
| Size: | 3061 bp |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Anhydrotetracycline
Please note that this product will be discontinued at the end of 2026.
Competent E. coli TOP10
Please note that this product will be discontinued at the end of 2026.
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pASK-IBA4C vector
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pASG-IBA2 vector
Please note that this product will be discontinued at the end of 2026.
pASG-IBA4 vector
Please note that this product will be discontinued at the end of 2026.
