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2-1325-000
pASK-IBA6C vector
Please note that this product will be discontinued at the end of 2026.
Please note that this product will be discontinued at the end of 2026.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tag®II can be removed by the protease factor Xa.
| Affinity Tag N'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using e.g. restriction enzyme BsaI in MCS |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Expression plasmid for Strep-tag®II fusion proteins |
| Promoter: | tet Promoter |
| Resistance: | Chloramphenicol |
| Sequence: | See Documents |
| Size: | 3081 bp |
| Specificity: | Factor Xa cleavage site |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Competent E. coli TOP10
Please note that this product will be discontinued at the end of 2026.
Anhydrotetracycline
Please note that this product will be discontinued at the end of 2026.
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Please note that this product will be discontinued at the end of 2026.
pASG-IBA4 vector
Please note that this product will be discontinued at the end of 2026.
