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2-1325-000
pASK-IBA6C vector
Periplasmic E. coli expression vector encoding an N-terminal Strep-tag®II, chloramphenicol resistance and protease cleavage site
Content
pASK-IBA6C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, and the sequence for a protease cleavage site.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tag®II can be removed by the protease factor Xa.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tag®II can be removed by the protease factor Xa.
| Affinity Tag N'-terminal: | Strep-tag®II |
|---|---|
| Cloning Method: | Direct cloning using e.g. restriction enzyme BsaI in MCS |
| Concentration: | 250 ng/µl |
| Expression Host: | E. coli |
| Form: | Suspension in TE buffer |
| Possible Application: | Expression plasmid for Strep-tag®II fusion proteins |
| Promoter: | tet Promoter |
| Resistance: | Chloramphenicol |
| Sequence: | See Documents |
| Size: | 3081 bp |
| Specificity: | Factor Xa cleavage site |
| Storage: | -20 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Accessories
Anhydrotetracycline
Derivative of tetracycline for induction of tetracycline-controlled promoters
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