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2-1503-001

Strep-Tactin® AP conjugate

Strep-Tactin® AP conjugate for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins

€127.00*

Content: 1 Stk
Content
The streptavidin variant Strep-Tactin® is available as conjugate with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag®II or Twin-Strep-tag® fusion proteins transferred to a blotting membrane. The AP conjugate is suitable for chromogenic reaction.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa ) of E. coli. Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag®II or Twin-Strep-tag® fusion proteins with Strep-Tactin® AP to prevent the detection of false positive bands. For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.

Specifications

Amount: 500 µl
Form: Suspension in PBS
Possible Application: Western blot, detection in microplates
Specificity: Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

GFP-Strep-tag®II control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
GFP-Twin-Strep-tag® control protein
Control protein for Strep-Tactin® and Strep-Tactin®XT applications
Strep-tag®II peptide
Synthetic peptide recognized by Strep-Tactin® and Strep-Tactin®XT

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