Strep-Tactin® Magnetic Microbeads
Strep-Tactin® multimers conjugated with magnetic microbeads
Please note: Strep-Tactin® Magnetic Microbeads were specifically developed for cell isolation. For protein purification, we offer MagStrep "type3" XT beads and MagStrep "type3" Strep-Tactin® beads, which have an agarose coating that ensures a high protein binding capacity.
Strep-Tactin® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin®. They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to the microbeads. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.
|Bead Size:||1 - 3 µm|
|Form:||Suspension in DPBS|
|Possible Application:||Cell isolation (magnetic)|
|Recommended Usage:||15 µl per 1 x 10^7 total cells|
|Storage:||2-8 °C; Do not freeze!|
|Stability:||6 months after shipping|
Protective T cell receptor identification for orthotopic reprogramming of immunity in refractory virus infections (Stief et al., 2022, Molecular Therapy)
Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments (Nannini et al., 2021, Monoclonal Antibodies)
Rab1-AMPylation by Legionella DrrA is allosterically activated by Rab1 (Du et al., 2021, Nature Communications)