6-5510-050
Strep-Tactin® Magnetic Microbeads
Strep-Tactin® multimers conjugated with magnetic microbeads
Quantity | Unit price |
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1-2 |
€215.50*
|
3-9 |
€205.00*
|
From 10 |
€182.50*
|
Content
Please note: Strep-Tactin® Magnetic Microbeads were specifically developed for cell isolation. For protein purification, we offer MagStrep "type3" XT beads and MagStrep "type3" Strep-Tactin® beads, which have an agarose coating that ensures a high protein binding capacity.
Strep-Tactin® Magnetic Microbeads are microspheres covalently linked to Strep-Tactin®. They are suitable for labeling antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®). To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to the microbeads.
Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting magnetic complexes can be used to label cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents permits serial positive selection as well as depletion steps and reserves the cells’ full biological activity.
Bead Size: | 1 - 3 µm |
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Form: | Suspension in DPBS |
Possible Application: | Cell isolation (magnetic) |
Recommended Usage: | 15 µl per 1 x 10^7 total cells |
Storage: | 2-8 °C; Do not freeze! |
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Stability: | 6 months after shipping |
Shipping: | Room temperature |