Specifications for Strep-Tactin® Purification Resins
There are several Strep-Tactin® resin versions available. All resins differ in their properties and suitability for applications. Learn more about the different resin matrices in order to know when to use which resin.
|Strep-Tactin® Superflow High capacity||Strep-Tactin®XT Superflow||Strep-Tactin®XT Superflow High capacity||Strep-Tactin®
MacroPrep (only available as 20 ml slurry)
|Gravity flow column||Yes||Yes||Yes||Yes||Yes||Yes|
|Binding capacity*||3 mg/ml resin||3 mg/ml resin||n. d.||n. d.||n. d.||3 mg/ml resin|
|Dynamic binding capacity**||n. d.||n. d.||7.0 mg/ml resin||4.1 mg/ml resin||15 mg/ml resin||n. d.|
|Bead support||Sepharose 4 FF||Superflow 6||Superflow 6||Superflow 6||Superflow 6||MacroPrep 50|
|Bead structure||4% agarose, crosslinked||6% agarose, crosslinked||6% agarose, crosslinked||6% agarose, crosslinked||6% agarose, crosslinked||polymethacrylate|
|Bead size||45 - 165 µm||60 - 160 µm spherical||60 - 160 µm spherical||60 - 160 µm spherical||60 - 160 µm spherical||50 µm|
|Exclusion limit||~3 x 107||6 x 106||6 x 106||6 x 106||6 x 106||1 x 106|
|Recommended flow rate||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min|
|pH range for protein binding||7-8||7-8||7-8||6-10||6-10||7-8|
|Max pressure||Gravity flow||9,6 bar||9,6 bar||9,6 bar||9,6 bar||70 bar|
|Form||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension|
|Storage||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze|
|* Max binding capacity for a Strep-tag®II protein (30kDa).
** Dynamic binding capacity (DBC) was determined with mCherry-Twin-Strep-tag® (30 kDa). The DBC is determined under realistic operation conditions and shows the amount of protein which is bound until 10% of the protein is found in the flow through.
For purification of Strep-tag®II proteins by gravity flow column chromatography
For packing individual columns bulk material is available.
For batch purification we recommend to use magnetic beads: MagStrep "type3" XT beads.
Both Strep-Tactin® Superflow® resins:
- combine outstanding flow characteristics with superior mechanical stability
- are suitable for increased flow rates and useful for purification of large protein complexes
- yield much higher protein purity (> 95 %) than 6xHistidine-tag purification on Ni-NTA Superflow®
Both resins are available as:
- 50 % suspension (slurry)
- pre-packed Strep-Tactin® gravity flow columns
- pre-packed cartridges for work with automated workstations like Äkta
Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the solution for Strep-tag® fusion proteins at high expression levels.
So far, Strep-Tactin® Superflow® high capacity is one of the most favored resins allowing the purification of recombinant proteins in high yields. However, the new Strep-Tactin®XT Superflow® high capacity provides significant more purified protein because it combines an increased capacity of the resin with a very efficient binding due to the high affinity for Strep-tag®II and Twin-Strep-tag®.
The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa.
MacroPrep® is available in one format: