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Strep-Tactin® purification resins

Several Strep-Tactin® resin versions ( Sepharose®, Superflow®, Superflow® high capacity and MacroPrep®) are available which differ in their properties and suitability for applications:

Read about the different provided resin matrices

When to use which resin?
Sepharose®
  • Economic alternative to Superflow®
  • Agarose support with good flow properties
  • For gravity flow chromatography only (not pressure stable)

Superflow®

  • Our all-rounder and the resin to start with for large proteins and protein:protein interaction analysis
  • Agarose support with low unspecific binding properties
  • For increased flow rates
  • Low pressure stable (FPLC/HPLC/Äkta applications)

Superflow® high capacity (HC)

  • The capacity of Superflow® is not sufficient or the binding affinity to Twin-/Strep-tag® fusion
  • proteins needs to be slightly improved!
  • 3-5 fold higher binding capacity as Superflow®
MacroPrep®
  • The alternative if Sepharose® and Superflow® lead to non-specific binding of protein (e.g. Chlorophyll from plant extracts)
  • Synthetic support with low non-specific binding propertie
  • Highly pressure stable (FPLC/HPLC /Äkta applications)

 

Strep-Tactin® Resin specifications
  Strep-Tactin® Sepharose® Strep-Tactin® Superflow®

Strep-Tactin® Superflow® High capacity (HC)

 Strep-Tactin® MacroPrep®
Gravity flow column
 Yes Yes Yes Yes
batch purification
 No
 No No
 No
FPLC No
 Yes Yes Yes
HPLC
 No
 Yes Yes Yes
Binding capacity
 50 - 100 nmol/ml 50 - 100 nmol/ml 150 - 500 nmol/ml 50 - 100 nmol/ml
Support Sepharose 4 FF
 Superflow 6
 Superflow 6
 MacroPrep 50
Bead structure
 agarose
 6% agarose, crosslinked
 6% agarose, crosslinked polymethacrylate
Bead size
 45 - 165 µm 60 - 160 µm spherical 60 - 160 µm spherical 50 µm
Exclusion limit
 3 x 107 6 x 106 6 x 106 1 x 106
Recommended linear flow rate* up to 30 cm/h up to 300 cm/h up to 300 cm/h up to 300 cm/h
pH stability 4-9
 2-13
 2-13
 2-12
Max pressure Gravity flow 9,6 bar 9,6 bar 70 bar (cartridge cover 20 bar only)
Form buffered 50% suspension buffered 50% suspension buffered 50% suspension buffered 50% suspension
Storage 4 °C, do not freeze 4 °C, do not freeze 4 °C, do not freeze 4 °C, do not freeze
Shipment RT
 RT
 RT
 RT
Strep-Tactin® Sepharose®

For purification of Strep-tag®II proteins by gravity flow column chromatography

Strep-Tactin® Sepharose® provides high binding capacity and minimal non-specific binding.
The material can be used for gravity flow purification. Since the Strep-tag® protein purification should be performed as column affinity chromatography, we recommend our pre-packed Strep-Tactin® Sepharose® gravity flow columns, which are available in different formats (from 0.2 ml mini columns up to 10 ml columns).

For packing individual columns bulk material is available.

For batch purification we recommend to use magnetic beads: MagStrep "type3" XT beads.

Strep-Tactin® Superflow®

Both Strep-Tactin® Superflow® resins:

  • combine outstanding flow characteristics with superior mechanical stability
  • are suitable for increased flow rates and useful for purification of large protein complexes
  • yield much higher protein purity (> 95 %) than 6xHistidine-tag purification on Ni-NTA Superflow®

Both resins are available as:

  • 50 % suspension (slurry)
  • pre-packed Strep-Tactin® gravity flow columns
  • pre-packed cartridges for work with automated workstations like Äkta
Strep-Tactin® Superflow® high capacity

Difference between Strep-Tactin® Superflow® and Strep-Tactin® Superflow® high capacity:

Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the perfect solution for Strep-tag® fusion proteins at high expression levels.

 

 

The Strep-Tactin® Superflow® high capacity resin

  • provides 2 to 3 times higher binding capacity than standard Strep-Tactin® Superflow® (150 – 500 nmol or up to 15 mg recombinant Strep-tag®II fusion protein per ml sedimented resin)
  • enables a cost effective purification of higher protein amounts at optimal purity
  • isolation of comparable protein yields with His-tag/Ni-NTA purification

 

 

Fig1: Comparison of Strep-Tactin® Superflow® with Ni-NTA Superflow® in respect to binding capacity and protein purity.

Strep-Tactin® MacroPrep®

The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa.

MacroPrep® is available in different formats:

  • Pre-packed gravity flow columns
  • Spin columns for rapid parallel purification of Strep-tag® proteins
  • Suspension/Slurry