Specifications for Strep-Tactin® Purification Resins
Several Strep-Tactin® resin versions ( Sepharose®, Superflow®, Superflow® high capacity and MacroPrep®) are available which differ in their properties and suitability for applications:
Read about the different provided resin matrices
Sepharose® |
|
Superflow® |
|
Superflow® high capacity (HC) |
|
MacroPrep® |
|
Strep-Tactin® Sepharose® | Strep-Tactin® Superflow® |
Strep-Tactin® Superflow® High capacity (HC) |
Strep-Tactin® MacroPrep® | |
Gravity flow column |
Yes | Yes | Yes | Yes |
batch purification |
No |
No | No |
No |
FPLC | No |
Yes | Yes | Yes |
HPLC |
No |
Yes | Yes | Yes |
Binding capacity |
50 - 100 nmol/ml | 50 - 100 nmol/ml | 150 - 500 nmol/ml | 50 - 100 nmol/ml |
Support | Sepharose 4 FF |
Superflow 6 |
Superflow 6 |
MacroPrep 50 |
Bead structure |
agarose |
6% agarose, crosslinked |
6% agarose, crosslinked | polymethacrylate |
Bead size |
45 - 165 µm | 60 - 160 µm spherical | 60 - 160 µm spherical | 50 µm |
Exclusion limit |
3 x 107 | 6 x 106 | 6 x 106 | 1 x 106 |
Recommended linear flow rate* | up to 30 cm/h | up to 300 cm/h | up to 300 cm/h | up to 300 cm/h |
pH stability | 4-9 |
2-13 |
2-13 |
2-12 |
Max pressure | Gravity flow | 9,6 bar | 9,6 bar | 70 bar (cartridge cover 20 bar only) |
Form | buffered 50% suspension | buffered 50% suspension | buffered 50% suspension | buffered 50% suspension |
Storage | 4 °C, do not freeze | 4 °C, do not freeze | 4 °C, do not freeze | 4 °C, do not freeze |
Shipment | RT |
RT |
RT |
RT |
For purification of Strep-tag®II proteins by gravity flow column chromatography
For packing individual columns bulk material is available.
For batch purification we recommend to use magnetic beads: MagStrep "type3" XT beads.
Both Strep-Tactin® Superflow® resins:
- combine outstanding flow characteristics with superior mechanical stability
- are suitable for increased flow rates and useful for purification of large protein complexes
- yield much higher protein purity (> 95 %) than 6xHistidine-tag purification on Ni-NTA Superflow®
Both resins are available as:
- 50 % suspension (slurry)
- pre-packed Strep-Tactin® gravity flow columns
- pre-packed cartridges for work with automated workstations like Äkta

of Strep-Tactin® Superflow® high capacity
(ST HC) with Strep- Tactin®XT Superflow®
high capacity (STXT HC)
Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the solution for Strep-tag® fusion proteins at high expression levels.
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NEW: Strep-Tactin®XT Superflow® high capacity
So far, Strep-Tactin® Superflow® high capacity is one of the most favored resins allowing the purification of recombinant proteins in high yields. However, the new Strep-Tactin®XT Superflow® high capacity provides significant more purified protein because it combines an increased capacity of the resin with a very efficient binding due to the high affinity for Strep-tag®II and Twin-Strep-tag®.
The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa.
MacroPrep® is available in one format:
- Suspension/Slurry