Specifications for Strep-Tactin® Purification Resins
There are several Strep-Tactin® resin versions available. All resins differ in their properties and suitability for applications. Learn more about the different resin matrices in order to know when to use which resin.
Superflow (only available as 50 ml slurry)
|Strep-Tactin® Superflow high capacity||Strep-Tactin®XT Superflow||Strep-Tactin®XT Superflow high capacity||Strep-Tactin®
MacroPrep (only available as 20 ml slurry)
|Gravity flow column||Yes||Yes||Yes||Yes||Yes||Yes|
|Binding capacity*||3 mg/ml resin||3 mg/ml resin||n. d.||n. d.||n. d.||3 mg/ml resin|
|Dynamic binding capacity**||n. d.||n. d.||7.0 mg/ml resin||4.1 mg/ml resin||15 mg/ml resin||n. d.|
|Bead support||Sepharose 4 FF||Superflow 6||Superflow 6||Superflow 6||Superflow 6||MacroPrep 50|
|Bead structure||4% agarose, crosslinked||6% agarose, crosslinked||6% agarose, crosslinked||6% agarose, crosslinked||6% agarose, crosslinked||polymethacrylate|
|Bead size||45 - 165 µm||60 - 160 µm spherical||60 - 160 µm spherical||60 - 160 µm spherical||60 - 160 µm spherical||50 µm|
|Exclusion limit||~3 x 107||6 x 106||6 x 106||6 x 106||6 x 106||1 x 106|
|Recommended flow rate||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min||0.5-1 ml/min|
|pH range for protein binding||7-8||7-8||7-8||6-10||6-10||7-8|
|Max pressure||Gravity flow||9,6 bar||9,6 bar||9,6 bar||9,6 bar||70 bar|
|Form||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension||buffered 50% suspension|
|Storage||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze||4°C, do not freeze|
|* Max binding capacity for a Strep-tag®II protein (30kDa).
** Dynamic binding capacity (DBC) was determined with mCherry-Twin-Strep-tag® (30 kDa). The DBC is determined under realistic operation conditions and shows the amount of protein which is bound until 10% of the protein is found in the flow through.
For purification of Strep-tag®II proteins by gravity flow column chromatography
For packing individual columns bulk material is available.
For batch purification we recommend to use magnetic beads: MagStrep "type3" XT beads.
Strep-Tactin® Superflow® resin:
- combines outstanding flow characteristics with superior mechanical stability
- is suitable for increased flow rates and useful for purification of large protein complexes
- yields much higher protein purity (> 95%) than 6xHistidine-tag purification on Ni-NTA Superflow®
Strep-Tactin® Superflow® resin is available as:
- 50% suspension (slurry)
Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the solution for Strep-tag® fusion proteins at high expression levels.
Strep-Tactin® Superflow® high capacity resin is available as:
50% suspension (slurry)
pre-packed Strep-Tactin® gravity flow columns
pre-packed cartridges for work with automated workstations like Äkta
So far, Strep-Tactin® Superflow® high capacity is one of the most favored resins allowing the purification of recombinant proteins in high yields. However, the new Strep-Tactin®XT Superflow® high capacity provides significant more purified protein because it combines an increased capacity of the resin with a very efficient binding due to the high affinity for Strep-tag®II and Twin-Strep-tag®.
The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa.
MacroPrep® is available in one format:
- 50% suspension (slurry)