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Specifications for Strep-Tactin® Purification Resins

There are several Strep-Tactin® resin versions available. All resins differ in their properties and suitability for applications. Learn more about the different resin matrices in order to know when to use which resin.

Resin specifications

  Strep-Tactin® 
Sepharose		 Strep-Tactin® 
Superflow (only available as 50 ml slurry)		 Strep-Tactin® Superflow high capacity  Strep-Tactin®XT Superflow Strep-Tactin®XT Superflow high capacity  Strep-Tactin® 
MacroPrep (only available as 20 ml slurry)
Gravity flow column Yes Yes Yes Yes Yes Yes
Batch purification No No No Yes Yes No
FPLC No Yes Yes Yes Yes Yes
HPLC No Yes Yes Yes Yes Yes
Binding capacity* 3 mg/ml resin 3 mg/ml resin n. d. n. d. n. d. 3 mg/ml resin
Dynamic binding capacity** n. d. n. d.  7.0 mg/ml resin 4.1 mg/ml resin 15 mg/ml resin n. d.
Bead support Sepharose 4 FF Superflow 6 Superflow 6 Superflow 6 Superflow 6 MacroPrep 50
Bead structure 4% agarose, crosslinked 6% agarose, crosslinked 6% agarose, crosslinked 6% agarose, crosslinked 6% agarose, crosslinked polymethacrylate
Bead size 45 - 165 µm 60 - 160 µm spherical 60 - 160 µm spherical 60 - 160 µm spherical 60 - 160 µm spherical 50 µm
Exclusion limit ~3 x 107 6 x 106 6 x 106 6 x 106 6 x 106 1 x 106
Recommended flow rate 0.5-1 ml/min  0.5-1 ml/min  0.5-1 ml/min  0.5-1 ml/min  0.5-1 ml/min  0.5-1 ml/min 
pH range for protein binding 7-8 7-8 7-8 6-10 6-10 7-8
Max pressure Gravity flow 9,6 bar 9,6 bar 9,6 bar 9,6 bar 70 bar
Form buffered 50% suspension buffered 50% suspension buffered 50% suspension buffered 50% suspension buffered 50% suspension buffered 50% suspension
Storage 4°C, do not freeze 4°C, do not freeze 4°C, do not freeze 4°C, do not freeze 4°C, do not freeze 4°C, do not freeze
Shipment RT RT RT RT RT RT
* Max binding capacity for a Strep-tag®II protein (30kDa).
** Dynamic binding capacity (DBC) was determined with mCherry-Twin-Strep-tag® (30 kDa). The DBC is determined under realistic operation conditions and shows the amount of protein which is bound until 10% of the protein is found in the flow through.
Strep-Tactin® Sepharose®

For purification of Strep-tag®II proteins by gravity flow column chromatography

Strep-Tactin® Sepharose® provides high binding capacity and minimal non-specific binding.
The material can be used for gravity flow purification. Since the Strep-tag® protein purification should be performed as column affinity chromatography, we recommend our pre-packed Strep-Tactin® Sepharose® gravity flow columns, which are available in different formats (from 0.2 ml mini columns up to 10 ml columns).

For packing individual columns bulk material is available.

For batch purification we recommend to use magnetic beads: MagStrep "type3" XT beads.

Strep-Tactin® Superflow®

Strep-Tactin® Superflow® resin:

  • combines outstanding flow characteristics with superior mechanical stability
  • is suitable for increased flow rates and useful for purification of large protein complexes
  • yields much higher protein purity (> 95%) than 6xHistidine-tag purification on Ni-NTA Superflow®

Strep-Tactin® Superflow® resin is available as:

  • 50% suspension (slurry)
Strep-Tactin® Superflow® high capacity
Comparison of the dynamic binding capacity of Strep-Tactin® Superflow® high capacity (ST HC) with Strep- Tactin®XT Superflow® high capacity (STXT HC)
Comparison of the dynamic binding capacity
of Strep-Tactin® Superflow® high capacity
(ST HC) with Strep- Tactin®XT Superflow®
high capacity (STXT HC)

Strep-Tactin® Superflow® high capacity resin offers a three times higher binding capacity at identical high protein purity. It is the solution for Strep-tag® fusion proteins at high expression levels.

Strep-Tactin® Superflow®  high capacity resin is available as:

  • 50% suspension (slurry)

  • pre-packed Strep-Tactin® gravity flow columns

  • pre-packed cartridges for work with automated workstations like Äkta

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NEW: Strep-Tactin®XT Superflow® high capacity

So far, Strep-Tactin® Superflow® high capacity is one of the most favored resins allowing the purification of recombinant proteins in high yields. However, the new Strep-Tactin®XT Superflow® high capacity provides significant more purified protein because it combines an increased capacity of the resin with a very efficient binding due to the high affinity for Strep-tag®II and Twin-Strep-tag®.

Strep-Tactin® MacroPrep®

The MacroPrep® resin exhibits non-specific binding properties differing from those of Sepharose® or Superflow® and can, thus, be recommended in cases where suboptimal results are obtained with Sepharose® or Superflow®, and vice versa.

MacroPrep® is available in one format:

  • 50% suspension (slurry)