Strep-Tactin®XT 4Flow® FPLC column
Strep-Tactin®XT 4Flow® FPLC columns are intendet for automated affinity purification using liquid chromatography devices like Äkta® (Cytiva). The FPLC columns contain a crosslinked agarose coupled to streptavidin variant Strep-Tactin®XT. The resin offers outstanding target purity (>95%) and high binding affinity. Therefore, it is recommended for all protein classes, especially for low abundant or challenging proteins. Because of the low agarose concentration (4%), it is particularly suitable for large target protein purification.
For higher protein yields, we recommend our Strep-Tactin®XT 4Flow® high capacity FPLC columns.
When working with multimeric proteins, we recommend using our Strep-Tactin® Superflow® high capacity FPLC columns instead, since the increased avidity might influence elution efficiency.
See our resins specifications for more information and recommendations.
Please note that the Strep-Tactin®XT 4Flow® cartridges have been renamed Strep-Tactin®XT 4Flow® FPLC columns.
|Bead Size:||50 - 150 µm spherical|
|Dynamic Protein Binding Capacity:||5 mg/ml resin|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-tag® and Strep-tag®II|
|Support:||4% agarose, crosslinked|
|Stability:||6 months after shipping|