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2-5024-001

Strep-Tactin®XT 4Flow® FPLC column

FPLC column to purify Strep-Tag®II or Twin-Strep-tag® fusion proteins
Quantity Unit price
1-9
€256.00*
From 10
€205.00*
Content

Strep-Tactin®XT 4Flow® FPLC columns are intendet for automated affinity purification using liquid chromatography devices like Äkta® (Cytiva). The FPLC columns contain a crosslinked agarose coupled to streptavidin variant Strep-Tactin®XT. The resin offers outstanding target purity (>95%) and high binding affinity. Therefore, it is recommended for all protein classes, especially for low abundant or challenging proteins. Because of the low agarose concentration (4%), it is particularly suitable for large target protein purification.

For higher protein yields, we recommend our Strep-Tactin®XT 4Flow® high capacity FPLC columns.

When working with multimeric proteins, we recommend using our Strep-Tactin® Superflow® high capacity FPLC columns instead, since the increased avidity might influence elution efficiency.

See our resins specifications for more information and recommendations.

Please note that the Strep-Tactin®XT 4Flow® cartridges have been renamed Strep-Tactin®XT 4Flow® FPLC columns.

Specifications

Bead Size: 50 - 150 µm spherical
Dynamic Protein Binding Capacity: 5 mg/ml resin
Form: Pre-packed column
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Buffer W (10x)
Strep-Tactin® and Strep-Tactin®XT wash buffer
Buffer BXT (10x)
Strep-Tactin®XT elution buffer
Biotin
Biotin powder for preparation of Strep-Tactin®XT elution buffer
Buffer XT-R
Strep-Tactin®XT regeneration buffer
Strep-Tactin®XT Buffer Set
Strep-Tactin®XT purification buffer set

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Strep-Tactin®XT 4Flow® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® resin
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MagStrep® Strep-Tactin®XT beads
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Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
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Empty Spin Columns
For rapid protein purification with Strep-Tactin®XT 4Flow® high capacity
Strep-Tactin®XT 4Flow® high capacity FPLC column
FPLC column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins