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2-5151-000

Strep-Tactin®XT 4Flow® high capacity Spin Column Kit

For rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins

€270.00*

Content
Please be informed that we have replaced the Strep-Tactin®XT Spin Column Kit (2-4151-000) by our new improved kit with higher protein binding capacity, Strep-Tactin®XT 4Flow® high capacity Spin Column Kit.

Strep-Tactin®XT 4Flow® high capacity Spin Column Kit is perfectly suitable for rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins from multiple small samples in parallel. The kit contains ready-to-use wash buffer as well as elution buffer, Strep-Tactin®XT 4Flow® high capacity resin (50% suspension) and empty spin columns. The spin columns are not prefilled with resin to allow a customer-specific application and experimental design. If the spin columns are filled with, e.g., 100 µl Strep-Tactin®XT 4Flow® high capacity resin (50 µl column bed volume) up to 600 µg protein can be purified. The frit (mesh size 10 µm) in the lower part of the empty spin columns ensure that the resin remain in the spin column while sample or buffer flow through rapidly during the centrifugation process. The spin columns are compatible with conventional 1.5 ml or 2.0 ml reaction tubes. The maximum filling volume is 700 µl.

Specifications

Binding Capacity: High capacity
Detector/Ligand: Strep-Tactin®XT
Format: Kit
Kit Content: 40 empty spin columns, once 1x Buffer W and 1x Buffer BXT
Matrices: 4Flow®
Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-tag® and Strep-tag®II
Support: 4% agarose, crosslinked
Twin-Strep-tag® Binding Capacity: 600 µg/50 µl resin bed

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

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Strep-Tactin®XT 4Flow® resin
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