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Immobilization with Strep-Tactin®XT:Twin-Strep-tag®

Due to the extra tight binding affinity of Strep-Tactin®XT to Twin-Strep-tag®, the system is highly suitable for efficient immobilization of Twin-Strep-tag® (and Strep-tag®II) fusion proteins.

The binding affinity is in the range of antibody based immobilization system, but more economic and straight-forward to use.

New applications of the Strep-tag® system and key features
New applications: Excellent performance:
  • BIAcore
  • Enzyme activity assays
  • Diagnostic assays
  • High affinity in pM range (T1/2 = 13 h)
  • Reversibility due to addition of Biotin
  • Minimal non-specific binding


Develop your own Assay!

Due to the high binding affinity of Strep-Tactin®XT with Twin-Strep-tag® in the low pM range, it is highly suitable for assays like SPR (surface plasmon resonance).

Request unconjugated Strep-Tactin®XT using this form:

Strep-Tactin®XT request


Strep-Tactin®XT immobilization plates
  • Ready-to-use 96-well plates
  • Minimal non-specific binding
  • Minimal coefficients of variation
  • High affinity (pM range)
  • Reversibility maintaines upon addition of biotin
  • Cost effective

Strep-Tactin®XT Immobilization Products

Product Contents Cat.no Price   Order
Strep-Tactin® XT coated microplate 1 plate 2-4101-001 USD 60.00

Ready-to-use microplates (12 x 8-well strips) for diagnostic/assay applications, coated with Strep-Tactin® XT, a new Strep-Tactin variant with higher affinity for Strep-tag® II fusion proteins (nM affinity) and especially Twin-Strep-tag fusion proteins (pM affinity). Fusion proteins are selectively captured from complex mixtures and the biomolecules of interest are presented to interacting partners in a uniform manner which results in reliable and reproducible assays. The solid-phase, ...

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