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Immobilization with Strep-Tactin®XT:Twin-Strep-tag®

Due to the extra tight binding affinity of Strep-Tactin®XT to Twin-Strep-tag®, the system is highly suitable for efficient immobilization of Twin-Strep-tag® (and Strep-tag®II) fusion proteins.

The binding affinity is in the range of antibody based immobilization system, but more economic and straight-forward to use.

Key benefits of Strep-Tactin®XT and Twin-Strep-tag® for immobilization experiments

  • High affinity in pM range (T1/2 = 13 h)
  • Reversibility due to addition of Biotin
  • Minimal non-specific binding

Strep-Tactin®XT Immobilization Products

Product Contents Cat.no Price   Order
Strep-Tactin® XT coated microplate 1 plate 2-4101-001 USD 60.00

Ready-to-use microplates (12 x 8-well strips) for diagnostic/assay applications, coated with Strep-Tactin® XT, a new Strep-Tactin variant with higher affinity for Strep-tag® II fusion proteins (nM affinity) and especially Twin-Strep-tag fusion proteins (pM affinity). Fusion proteins are selectively captured from complex mixtures and the biomolecules of interest are presented to interacting partners in a uniform manner which results in reliable and reproducible assays. The solid-phase, ...

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Strep-Tactin®XT immobilization plates

  • Ready-to-use 96-well plates
  • Minimal non-specific binding
  • Minimal coefficients of variation
  • High affinity (pM range)
  • Reversibility maintaines upon addition of biotin
  • Cost effective

Application examples for Strep-Tactin®XT immobilization plates

The following assay shows the comparison of the immobilization efficiency of the bacterial alkaline phosphatase (BAP-Strep-tag®II) on Strep-Tactin®XT and Strep-Tactin®. Here, different amounts of BAP were subjected to the coated microplates. After washing, the relative maximum amount of immobilized BAP was determined. The relative signal intensity reflects the amount of bound BAP.

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