StrepMAB-Immo DY-549 is a murine, high-affinity monoclonal antibody, which is especially suited for detection of Strep-tag®II and Twin-Strep-tag®
fusion proteins in applications like western blots
, immunohistochemistry and immunocytochemistry
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
The absorption/emission maxima of the fluorescent dye is:
• DY-549: 550-560/575 nm
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.