2-1567-050

StrepMAB-Immo DY-549 conjugate

Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins

Quantity	Unit price
1-1	€291.00*
2-4	€276.00*
From 5	€261.50*

Content

100 µl

StrepMAB-Immo DY-549 is a murine, high-affinity monoclonal antibody, which is especially suited for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins in applications like western blots, ELISA, immunohistochemistry and immunocytochemistry.

The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).

The absorption/emission maxima of the fluorescent dye is:

• DY-549: 550-560/575 nm

Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.

Specifications

Clonality:	Monoclonal
Concentration:	0.5 mg/ml
Form:	Suspension in PBS
Possible Application:	FACS, Immunofluorescence, ELISA
Specificity:	Strep-tag®II and Twin-Strep-tag®
Subclass:	IgG1
Species:	Mouse