2-1567-050
StrepMAB-Immo DY-549 conjugate
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
Quantity | Unit price |
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1-1 |
€291.00*
|
2-4 |
€276.00*
|
From 5 |
€261.50*
|
Content
StrepMAB-Immo DY-549 is a murine, high-affinity monoclonal antibody, which is especially suited for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins in applications like western blots, ELISA, immunohistochemistry and immunocytochemistry.
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag®II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing Strep-tag®II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag® always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
The absorption/emission maxima of the fluorescent dye is:
• DY-549: 550-560/575 nm
Please note: For detection of Strep-tag®II or Twin-Strep-tag® fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin® is recommended.
Clonality: | Monoclonal |
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Concentration: | 0.5 mg/ml |
Form: | Suspension in PBS |
Possible Application: | FACS, Immunofluorescence, ELISA |
Specificity: | Strep-tag®II and Twin-Strep-tag® |
Subclass: | IgG1 |
Species: | Mouse |
Storage: | 2-8 °C |
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Stability: | 6 months after shipping |
Shipping: | Room temperature |