StrepMAB-Immo DY-549 conjugate
Conjugated monoclonal antibody to detect Strep-tag®II and Twin-Strep-tag® fusion proteins
StrepMAB-Immo DY-549 is a murine monoclonal antibody conjugated to a fluorescent dye. It has a high affinity to Strep-tag® and Twin-Strep-tag® fusion proteins, and can be used for detection in ELISA, FACS, immunohistochemistry and immunocytochemistry.
For proteins carrying the Strep-tag® with an N-terminal Serine-Alanine linker (protein-SA-WSHPQFEK or SA-WSHPQFEK-protein), the binding affinity is in the pM range. Proteins with other N-terminal sequences bind with a reduced affinity. The Twin-Strep-tag® includes an N-terminal SA-linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK).
The absorption/emission maxima of the fluorescent dye is:
• DY-549: 550-560/575 nm
For use in Western Blots, we recommend Strep-Tactin®, Strep-Tactin®XT or StrepMAB-Classic conjugates.
|Form:||Suspension in PBS|
|Possible Application:||FACS, Immunofluorescence, ELISA|
|Specificity:||Strep-tag®II and Twin-Strep-tag®|
|Stability:||6 months after shipping|