2-1000-025

10x Buffer E

Strep-Tactin® elution buffer with desthiobiotin

€50.00*

Content
10x Buffer E is a 10x concentrated Strep-Tactin® elution buffer. The contained desthiobiotin is a modified form of biotin that displaces Strep-tag®II and Twin-Strep-tag® fusion proteins from the modified biotin-binding pocket of Strep-Tactin® in a competitive manner, resulting in a mild elution. Please note that 10x Buffer E is not applicable for elution from Strep-Tactin®XT resins.

Specifications

Concentration: 10x concentrated
Form: Solution
Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin® resins

Shipping information

Storage: 2-8 °C
Stability: 12 months after shipping
Shipping: Room temperature

Accessories

Strep-Tactin® Sepharose® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Sepharose® column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column
Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity cartridge
HPLC/FPLC cartridge to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® MacroPrep® resin
50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

Related Products

Desthiobiotin
Desthiobiotin powder for preparation of Strep-Tactin® elution buffer
Strep-Tactin® Buffer Set
Strep-Tactin® purification buffer set
10x Buffer W
Strep-Tactin® and Strep-Tactin®XT wash buffer
10x Buffer R
Strep-Tactin® regeneration buffer with HABA