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6-8015-150

CD81 Fab-Strep, human

Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD81
Content
CD81 Fab-Strep is a recombinantly expressed low affinity Fab fragment fused to a Twin-Strep-tag®. Fab-Streps were developed for Traceless Affinity Cell Selection (Fab-TACS®), which is based on our Strep-tag® technology. Due to their low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell or exosome surface. Combining Fab-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells or alternatively, also to exosomes.

Depending on the Strep-Tactin® backbone, the following applications are possible:
• Fluorescent cell staining & sorting: Fab-Streps are combined with fluorophore-conjugated Strep-Tactin®.
• Affinity chromatographic cell & exosome isolation: Fab-Streps are loaded in Strep-Tactin® TACS Agarose columns.

By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target populations in all applications, yielding minimally manipulated cells or exosomes for unbiased further use.

Specifications

Endotoxin Level: ≤ 0.1 EU/µg by LAL test
Form: Lyophilized
Possible Application: Fluorescent cell staining & sorting, affinity chromatographic cell & exosome isolation
Purity: ≥ 90%
Reconstitution: Reconstitute in 1 ml buffer, e.g. 1x PBS containing 1 mM EDTA and 0.5% BSA, for a final concentration of 50 µg/ml.
Accessories: Fab-Streps

Shipping information

Storage: 2-8 °C; reconstituted: -80 °C
Stability: 6 months after shipping
Shipping: Room temperature or blue ice

Accessories

Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
Strep-Tactin® PE
Fluorophore-conjugated Strep-Tactin® for cell staining
Strep-Tactin® TACS Agarose
50% suspension for traceless affinity cell and exosome isolation