6-5000-001

Strep-Tactin® PE

Fluorophore-conjugated Strep-Tactin® for cell staining
Quantity Unit price
1-2
€293.00*
3-9
€278.00*
From 10
€249.00*
Content
Strep-Tactin® conjugated to  R-Phycoerythrin (PE) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®) based on our Strep-tag® technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells’ full biological activity.

Specifications

Form: Suspension in DPBS
Possible Application: Fluorescent cell staining & sorting
Recommended Usage: 1 µl per 2 x 10^6 (MHC I-Streps) or 5 x 10^6 (Fab-Streps) total cells

Shipping information

Storage: 2-8 °C; Do not freeze!
Stability: 12 months after shipping
Shipping: Blue ice

Accessories

MHC I-Strep HLA-A*0201; CMV pp65 (NLVPMVATV)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
MHC I-Strep HLA-A*0201; SARS-CoV-2 (YLQPRTFLL)
MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
CD3 Fab-Strep, human
Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD3
10x Buffer CI
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA

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Gravity flow column suitable for traceless affinity cell and exosome isolation
Strep-Tactin® TACS Agarose
50% suspension for traceless affinity cell and exosome isolation