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6-6310-300

Strep-Tactin® TACS Agarose Column

Gravity flow column suitable for traceless affinity cell and exosome isolation
Quantity Unit price
1-4
€45.00*
5-9
€39.00*
From 10
€35.00*
Content
Strep-Tactin® TACS Agarose columns are ready-to-use columns for cell selection or depletion via affinity chromatography. They contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the optimal use for cell-related approaches. 
The columns can be loaded with a protein of choice that is fused to a Twin-Strep-tag®. This can be for example an antibody or an antigen, which captures the cells inside the columns. Biotin addition causes the dissociation of strep-tagged proteins from Strep-Tactin®, leading to the elution of cells.
Alternatively, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated proteins (irreversible binding) for negative cell selection or depletion. 

Specifications

Cell Binding Capacity: 2 x 10^7 (0.3 ml) or 1 x 10^8 (1 ml) target cells
Form: Pre-packed in buffer
Possible Application: Cell isolation or depletion via affinity chromatography
Support: Crosslinked agarose

Shipping information

Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature

Accessories

Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
Buffer CI (10x)
Cell isolation buffer containing 10x PBS with 10 mM EDTA and 5% BSA
TACS Column Adapter
Adapter for Strep-Tactin® TACS Agarose columns that fits onto standard tubes

Related Products

Strep-Tactin® TACS Agarose
50% suspension for traceless affinity cell and exosome isolation
Strep-Tactin® PE
Fluorophore-conjugated Strep-Tactin® for cell staining
Strep-Tactin® APC
Fluorophore-conjugated Strep-Tactin® for cell staining