6-6310-300
Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
Strep-Tactin® TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The cells are eluted and ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes).
Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin® TACS Agarose columns also suitable for negative cell selection or depletion approaches.
| Cell Binding Capacity: | 2 x 10^7 (0.3 ml) or 1 x 10^8 (1 ml) target cells |
|---|---|
| Form: | Pre-packed in buffer |
| Possible Application: | Affinity chromatographic cell or exosome isolation |
| Support: | Crosslinked agarose |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Data Sheet
Example protocol: Isolation of human CD3+ T cells from PBMCs, whole blood or buffy coat (1 ml column)
Example protocol: Isolation of murine CD4+ T cells from splenocytes (0.3 ml column)
Protocol for Fab-TACS® affinity chromatographic exosome isolation (1 ml column)
MSDS Strep-Tactin® TACS Agarose
Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Enable a Stable Non-Spilling Loading of T Cells and Their Magnetic Accumulation (Boosz, P. et al., 2021, Cancers)
5-Aza-2-deoxycytidine alleviates the progression of primary biliary cholangitis by suppressing the FoxP3 methylation and promoting the Treg/ Th17 balance (Jiang, T. et al., 2021, International Immunopharmacology)
Loading of Primary Human T Lymphocytes with Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Does Not Impair Their Activation after Polyclonal Stimulation. (Mühlberger, M., et al., 2020, Cells)
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