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Strep-Tactin® TACS Agarose Column
Gravity flow column suitable for cell and exosome isolation via affinity chromatography
Strep-Tactin® TACS Agarose columns are ready-to-use columns for cell selection or depletion via affinity chromatography. They contain agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the optimal use for cell-related approaches.
The columns can be loaded with a protein of choice that is fused to a Twin-Strep-tag®. This can be for example an antibody or an antigen, which captures the cells inside the columns. Biotin addition causes the dissociation of strep-tagged proteins from Strep-Tactin®, leading to the elution of cells.
Alternatively, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated proteins (irreversible binding) for negative cell selection or depletion.
| Cell Binding Capacity: | 2 x 10^7 (0.3 ml) or 1 x 10^8 (1 ml) target cells |
|---|---|
| Form: | Pre-packed in buffer |
| Possible Application: | Cell isolation or depletion via affinity chromatography |
| Support: | Crosslinked agarose |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
Accessories
Biotin stock solution
100 mM concentrated biotin for dissociation of Twin-Strep-tag® from Strep-Tactin® suitable for cells
TACS Column Adapter
Adapter for Strep-Tactin® TACS Agarose columns that fits onto standard tubes
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Strep-Tactin® TACS Agarose
50% suspension for isolation of cells and exosomes via affinity chromatography
