Vectors for Entry and Fusion Cloning

Find a list with available vecotrs for entry or fusion cloning of recombinant target proteins below.

More information on this cloning strategies are given in section "strategies".

Overview on the Fusion Cloning Procedure

StarGate fusion cloning provides the possibility to express two or more GOIs from only one Acceptor Vector. The GOIs, which are present in respective Donor Vectors are transferred to the pNFUSE-IBA and the pCFUSE-IBA vectors. The GOI, which will be located upstream, needs to be conveyed to the pNFUSE-IBA vector, this vector contains the intergenic region. The downstream located GOI is cloned into pCFUSE-IBA. The transfer is unidirectional and oriented insertion of the gene is ensured.

In a next step, both genes are assembled in the Entry Vector using LguI restriction enzyme.

The resulting Donor Vector provides again Esp3I recognition sites for the transfer of the expression cassette (containing both GOIs) into the Acceptor Vector.

Product Contents Cat.no Price   Order
Reverse sequencing primer for pENTRY-IBA vectors (HPLC-purified) 1 nmol 5-0000-152 USD 26.00  
ENTRY-Primer-for2 Forward Sequencing Primer pENTRY 1 nmol 5-0000-153 USD 26.00  
Forward and Reverse sequencing primers for pENTRY-IBA vectors (HPLC-purified) 1 nmol each 5-0000-154 USD 34.00  
Forward sequencing primer for FUSION vectors (pCFUSE/pNFUSE) 1 nmol 5-0000-155 USD 26.00  
Reverse sequencing primer for FUSION vectors (pCFUSE/pNFUSE) 1 nmol 5-0000-156 USD 26.00  
Forward and Reverse sequencing primers for vectors (pCFUSE/pNFUSE) 1 nmol each 5-0000-157 USD 34.00  
Competent cells E. coli TOP10 20 rxns 5-1600-020 USD 108.00  

One-shot chemically competent E.coli TOP10 cells, 20reactions. ...

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StarGate Fusion Vector pCFUSE-IBA-11 5µg 5-1630-001 USD 260.00  

Downstream positioning of the inserted GOI in the final Fusion Donor vector ...

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StarGate Fusion Vector pNFUSE-IBA-IRES11 5µg 5-1631-001 USD 260.00  
 

. Upstream positioning of the inserted GOI in the final Fusion Donor vector ...

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StarGate Fusion Vector pNFUSE-IBA-SD11 5µg 5-1632-001 USD 260.00  

. Upstream positioning of the inserted GOI in the final Fusion Donor vector ...

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StarGate Fusion Vector pNFUSE-IBA-LINK11 5µg 5-1633-001 USD 260.00  

. Upstream positioning of the inserted GOI in the final Fusion Donor vector ...

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StarGate Fusion Vector pNFUSE-IBA-LINK12 5µg 5-1634-001 USD 260.00  

. Upstream positioning of the inserted GOI in the final Fusion Donor vector ...

Further details, manuals, data sheets, related products and more