Vectors for Entry and Fusion Cloning
Find a list with available vectors for entry or fusion cloning of recombinant target proteins below.
More information on this cloning strategies are given in section "strategies".
StarGate fusion cloning provides the possibility to express two or more GOIs from only one Acceptor Vector. The GOIs, which are present in respective Donor Vectors are transferred to the pNFUSE-IBA and the pCFUSE-IBA vectors. The GOI, which will be located upstream, needs to be conveyed to the pNFUSE-IBA vector, this vector contains the intergenic region. The downstream located GOI is cloned into pCFUSE-IBA. The transfer is unidirectional and oriented insertion of the gene is ensured.
In a next step, both genes are assembled in the Entry Vector using LguI restriction enzyme.
The resulting Donor Vector provides again Esp3I recognition sites for the transfer of the expression cassette (containing both GOIs) into the Acceptor Vector.
|Reverse sequencing primer for pENTRY-IBA vectors (HPLC-purified)||1 nmol||5-0000-152||USD 26.00|
|ENTRY-Primer-for2 Forward Sequencing Primer pENTRY||1 nmol||5-0000-153||USD 26.00|
replaces Cat. No. 5-0000-151
|Forward and Reverse sequencing primers for pENTRY-IBA vectors (HPLC-purified)||1 nmol each||5-0000-154||USD 34.00|
|Forward sequencing primer for FUSION vectors (pCFUSE/pNFUSE)||1 nmol||5-0000-155||USD 26.00|
|Reverse sequencing primer for FUSION vectors (pCFUSE/pNFUSE)||1 nmol||5-0000-156||USD 26.00|
|Forward and Reverse sequencing primers for vectors (pCFUSE/pNFUSE)||1 nmol each||5-0000-157||USD 34.00|
|Competent cells E. coli TOP10||20 rxns||5-1600-020||USD 108.00|
One-shot chemically competent E.coli TOP10 cells, 20reactions. ...
|Fusion Vector pCFUSE-IBA-11||5 µg||5-1630-001||USD 260.00|
Downstream positioning of the inserted GOI in the final Fusion Donor vector ...
|StarGate Fusion Vector pNFUSE-IBA-IRES11||5 µg||5-1631-001||USD 260.00|
|StarGate Fusion Vector pNFUSE-IBA-SD11||5 µg||5-1632-001||USD 260.00|
|StarGate Fusion Vector pNFUSE-IBA-LINK11||5 µg||5-1633-001||USD 260.00|
|StarGate Fusion Vector pNFUSE-IBA-LINK12||5 µg||5-1634-001||USD 260.00|