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The pASG-IBA104 vector allows the expression of Twin-Strep-tag®-fusion-proteins in [i]E.coli[/i]. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the Twin-Strep-tag® for N-terminal fusion to the recombinant protein and the Ampicillin Resistance cassette. It can be used with any [i]E. coli[/i] strain because the tet-promoter works independently from the genetic background of [i]E.coli[/i].
| Affinitätstag (N-terminal): | Twin-Strep-tag® |
|---|---|
| Expressionswirt: | E. coli |
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor for rekombinante Expression in E. coli |
| Plasmidgröße: | 3997 bp |
| Promoter: | tet Promoter |
| Resistenz: | Ampicillin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |
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pASG-IBA102 vector
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