The pESG-IBAwt2 vector allows the high-level stable and non-replicative transient expression of control proteins without tag in most mammalian hosts. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells, the BM40 signal for secretion of the recombinant protein as well as the Ampicillin resistance cassette for selection in [i]E.coli[/i] and the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be secreted into the medium.
| Expressionswirt: | Mammaliazellen |
|---|---|
| Form: | Suspension in TE Puffer |
| Klonierungsmethode: | Direkte Klonierung mit Restriktionsenzym Esp3I |
| Konzentration: | 250 ng/µl |
| Mögliche Anwendungen: | Vektor für rekombinante Expression in Mammaliazellen |
| Plasmidgröße: | 6115 bp |
| Promoter: | CMV Promoter |
| Resistenz: | Ampicillin, Neomycin |
| Sequenz: | Siehe Dokumente |
| Haltbarkeit: | 12 Monate nach Versand |
|---|---|
| Lagerung: | -20 °C |
| Versand: | Raumtemperatur |