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Strep-Tactin®XT 4Flow® high capacity resin
Strep-Tactin®XT 4Flow® high capacity is perfectly suited for purifying Strep-tag®II or Twin-Strep-tag® fusion proteins. This resin offers excellent pressure stability, pH compatibility, and target purity (> 95%). Additionally, this resin is compatible with a broad range of reagents.
Its high binding affinity makes it ideal for low abundant, high-expressing and challenging proteins. A low concentration of agarose also facilitates the purification of large proteins (>90 kDa). When working with, for example, multimeric proteins, choosing a resin with the Strep-Tactin® ligand is recommended.
Strep-Tactin®XT 4Flow® high capacity resin has a higher density of immobilized Strep-Tactin®XT than the classic Strep-Tactin®XT 4Flow®and thus can bind more protein.
As the resin is optimized for column affinity chromatography, rather than batch purification, the suspension can be used to pack gravity flow columns.
If you want more information, check out our resin specification overview.
| Bead Size: | 50 - 150 µm spherical |
|---|---|
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Protein Binding Capacity: | 31 mg/ml resin |
| Specificity: | Twin-Strep-tag® and Strep-tag®II |
| Support: | 4% agarose, crosslinked |
| Storage: | 2-8 °C |
|---|---|
| Stability: | 12 months after shipping |
| Shipping: | Room temperature |
Application Note
Application example
Video - Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared
Video - Packing of a Gravity Flow Column for Protein Purification
Read more about the Strep-Tactin®XT purification cycle
Read more about Strep-Tactin®XT resin specifications
Accessories
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