Strep-Tactin® Sepharose® is used preferentially for gravity flow purification of recombinant Strep-tag® proteins. Bulk resin is recommended for packing of columns since the resin is optimized for column affinity chromatography rather than for batch purification.
Binding capacity: 1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) is useful for the one-step purification of 50 to 100 nmol recombinant Strep-tag® protein (up to 3 mg in case of a 30 KDa protein).